川芎及两种主要药效成分对废用性肌萎缩的影响  

Effect of Ligusticum and its two main components on muscle atrophy in a hind limb unloaded rat model

作　　者：曹晋[1] 高云芳[1] 刘坤[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《中国应用生理学杂志》2010年第1期109-113,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金(30770273);陕西省教育厅科研计划项目(07JK382);陕西省中医管理局2007年度中医药科学技术研究专项(20072202)

摘　　要：目的:探讨川芎及川芎中起活血作用的两种主要药效成分(阿魏酸钠和川芎嗪)对后肢去负荷大鼠比目鱼肌萎缩的影响与作用。方法:尾部悬吊法建立大鼠废用性肌萎缩模型,用免疫组化技术及血液流变学方法观察药物对比目鱼肌各项指标的影响。结果:与后肢去负荷大鼠相比①高剂量的阿魏酸钠和川芎嗪使比目鱼肌I型肌纤维横截面积分别增加了37.3%和39.4%(P<0.05);②三种药物均能明显抑制梭外肌纤维MHCII表达水平的升高(P<0.01);③使肌梭内核袋2纤维MHCII的表达由阳性转变为阴性;④并能明显降低低切变率下的全血粘度。结论:川芎及两种主要药效成分阿魏酸钠与川芎嗪均能不同程度地对抗废用性肌萎缩的发生,以高剂量川芎嗪与阿魏酸钠的药效最为明显。Objective：To study the effects of Ligusticum and its two components（Sodium Ferulate and Ligustrazine as main efficacy components in Ligusticum for invigorating blood circulation） on muscle atrophy in a hind limb unloaded rat model.Methods：The tail-suspended rats were subjected to a 14-days disuse,immunohistochemistry and hemorheology were used to study the effects of medicines on soleus muscle.Results：Compared with HLU＋W：①The CSA of soleus type I fibers in HLU＋SfH and HLU＋TmpH increased by 37.3% and 39.4% respectively （P〈0.05）.②Expression level of MHC II were inhibited in all treatment groups（P〈0.01）.③Expression of MHC II in nuclear bag 2 fiber were altered from positive to negative.④The blood viscosity in low shear rates decreased obviously（P〈0.01）,even near to control.Conclusion：Ligusticum and its two main efficacy components（Sodium Ferulate and Ligustrazine） can prevent soleus atrophy induced by disuse,and Sodium Ferulate and Ligustrazine in high dose showed most efficacy.

关 键 词：川芎 废用性肌萎缩 肌球蛋白重链 肌梭 血流变 

分 类 号：R746.4[医药卫生—神经病学与精神病学]