牛去核卵母细胞能够被孤雌激活并发育到桑椹胚  

Derivation Morula from Bovine Enucleated Oocytes Activated by Parthenogenesis

作　　者：王志伟[1] 胡勇策[1] 马晓玲[1] 刘文强[1] 殷吉庆[1] 云彦[1] 雷安民[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西省干细胞工程技术研究中心,陕西省农业分子生物学重点实验室,杨凌712100

出　　处：《农业生物技术学报》2010年第1期87-91,共5页Journal of Agricultural Biotechnology

基　　金：西北农林科技大学留学回国人员科研启动项目(No.Z111020723)资助

摘　　要：为了探讨实验牛去核卵母细胞能否被激活,及其激活后的发育能力,本研究利用化学激活法对牛去核卵母细胞进行孤雌激活,然后在体外颗粒细胞饲养层上进行培养,同时在不同的时间采用免疫荧光技术对α-微管蛋白(α-tubulin)进行染色,以观察去核卵母细胞与正常卵母细胞中微管分布动态变化的差别。结果表明去核卵母细胞经激活后在体外培养能够发育到桑椹胚,去核卵母细胞内α-tubulin的分布在分裂初期没有分区现象,对照组未去核卵母细胞内的α-tubulin抗体出现了分区现象。The purpose of this study was to explore whether the bovine enucleated oocytes could be activated and to test their capacity of development after activation.We activated the enucleated oocytes using chemical methods,and cultured the embryos with granulosa cell feeder layer in vitro.To observe dynamic changes of microtubules in the enucleated and normal oocytes,we dyed the α-tubulin using immunofluorescence technology at different stages.The results showed that the enucleated oocytes could develop to morula after activation in vitro,and there was no phenomenon of zone division of α-tubulin in enucleated oocytes at initial phase,while the α-tubulin in the normal oocytes was distributed into several functional zones.

关 键 词：牛卵母细胞 Hoechst染色 激光共聚焦 

分 类 号：S828.3[农业科学—畜牧学]