产纤维素酶菌群的筛选及其酶学特性研究  

Isolation and enzymatic characteristics of cellulase producing microbial community

作　　者：宋安东[1] 王磊[1,2] 仉磊[2] 章晓庆[2] 杜然[2] 李十中[2] 

机构地区：[1]河南农业大学生命科学学院,郑州450002 [2]清华大学核能与新能源技术研究院,北京100084

出　　处：《中国生态农业学报》2010年第2期356-359,共4页Chinese Journal of Eco-Agriculture

基　　金：国家"十一五"科技支撑计划项目(2006BAD07A01)资助

摘　　要：从常年堆放的腐质豆秆中分离到1组产纤维素酶菌群MO, 并在50 ℃、pH8.0条件下培养, 90 h时达到最高酶活, 活性为1.361 1 IU。该酶最适反应温度为60 ℃, pH为7.0, 在60 ℃以下和pH3～8范围内具有良好的热稳定性和pH稳定性。在最适反应条件下, 该酶的最高活性可达2.13 IU。通过生长动力学研究了菌群内部不同生长阶段pH、酶活和失重率的相互关系, 并通过非变性电泳对酶谱进行初步研究, 得到7条活性条带, 说明MO中具有多种产酶菌株。A cellulase producing microbial community （MO） was isolated from rotted beanstalk compost. When the community was cultured at pH 8.0 and 50 ℃, maximal CMCase activity （1.361 1 IU） is observed in 90 hours. The optimal conditions for enzymatic reaction are 60 ℃ at pH 7.0. High heat and pH stability is observed at temperature lower than 60 ℃ and pH 3～8. CMCase activity of cellulase can reach 2.13 IU at optimal reaction point. Growth kinetics indicate some relationships among pH, CMCase activity and weight loss rate in different growth phases of microbial community. Through cellulase analysis for zymogram via native-PAGE, seven activity bands are observed. This suggests that more than one strain is responsible for the production of enzyme in MO.

关 键 词：纤维素酶 菌群 CMCase活性 非变性电泳 菌群动态变化 

分 类 号：Q93[生物学—微生物学]