固定化脂肪酶催化小桐子毛油合成生物柴油  

Synthesis of biodiesel from crude jatropha oil using immobilized lipase

作　　者：李俊奎[1] 王芳[1] 谭天伟[1] 邓利[1] 

机构地区：[1]北京化工大学北京市生物加工过程重点实验室,北京100029

出　　处：《北京化工大学学报（自然科学版）》2010年第2期100-103,共4页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：国家自然科学基金(20576013);国家"863"计划(2006AA020203);国家"973"计划(2007CB714304)

摘　　要：研究了以石油醚提取的小桐子毛油和甲醇为原料,利用固定化Candidasp.99-125脂肪酶催化反应合成生物柴油的可行性。考察了几种因素对酶法催化合成脂肪酸甲酯的影响。结果表明以小桐子毛油的质量为基准,在水含量为10%,溶剂量为2mL/g,脂肪酶量为20%,温度为40℃的条件下,3次流加甲醇,12 h后单批最高产率可达93%。在此条件下固定化酶连续使用14批,产率仍然可保持在70%以上。The enzymatic production of fatty acid methyl esters （FAMEs） from crude jatropha oil and methanol u- sing the immobilized lipase Candida sp. 99 - 125 has been studied. The crude oil was extracted by a simple method. The effect of varying water content, solvent content, enzyme amount, temperature and number of additions of methanol on the reaction were investigated. The optimal conditions for processing 1 g of crude jatropha oil were found to be： 10% water, 2 mL petroleum ether as solvent, 20% lipase based on the weight of oil, temperature 40 ℃ , and three-step addition of methanol. The maximum yield of FAMEs was 93% after 12h and after 14 cycles the yield of FAMEs was still above 70%.

关 键 词：生物柴油 小桐子毛油 固定化酶 

分 类 号：Q814.9[生物学—生物工程]