中肺合剂对放射性肺损伤大鼠血液中TGF-β1 IL-6及IL-1的影响  

The Effect of ZhongFeiHeJi Regulate the TGF-β1 IL-6 and IL-1 Expression in Peripheral Blood of Rats with Radioactive Injury of Lungs

作　　者：孙燕[1] 张爱琴[1] 马胜林[1] 包素珍[2] 冯建国[1] 

机构地区：[1]浙江省肿瘤医院,浙江杭州310022 [2]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2010年第3期571-573,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药管理局基金资助项目(2006Y024)

摘　　要：目的:探讨中肺合剂对放射性肺损伤大鼠血液中TGF-β1、IL-6及IL-1含量的影响。方法:90只Wistar大鼠随机取60只,建立大鼠放射性肺损伤模型:采用25Gy加速器射线单次全胸照射。模型建成后随机分为3组:正常组、模型组及中肺合剂组。中肺合剂组予中肺合剂进行治疗,8周后,腹主动脉取血,ELISA法检测血清中TGF-β1、IL-6、IL-1的含量。结果:与模型组比较,中肺合剂组大鼠的生存率并未得到提高(P=0.243),但血清中TGF-β1、IL-6明显降低(P<0.01),而IL-1则无明显变化(P>0.05)。结论:中肺合剂可通过调节细胞因子的分泌,改善大鼠的生活质量,进而减轻放射性肺损伤的程度。Objective：To research the effect that the ZhongFeiHeJi to influence the level of TGF–β1、IL-6 and IL-1 in peripheral blood of rats with radioactive injury of lungs.Methods：The rats in ZhongFeiHeJi group were poured with ZhongFeiHeJi after 90 Wistar rats were randomly divided into the normal group,the model group,the ZhongFeiHeJi group,and the rat model of radiation-induced pulmonary injury was copied by radiated with 6MV-X ray linear accelerator for 25Gy dose. After 8 weeks,the concentration of the cytokine such as TGF-β,IL-1,IL-6 in blood serum with the methods of enzyme-linked immunosorbent assay.Results：The survival rate of rats in the ZhongFeiHeJi group wasn＇t elevated than them in the model group,and the expressing level of TGF–β1 and IL-6 in the ZhongFeiHeJi group was lower than then in the model group（P0.01）,but the ZhongFeiHeJi group without obvious effect on the level of IL-1 compare with the model group.Conclusion：ZhongfeiHeJi can improve the live quality of rats by to regulating excreting of cytokine,and have the preventive and therapeutic effect on the rats with radioactive injury of lungs.

关 键 词：中肺合剂 放射性肺损伤 TNF-α TGF-Β1 IL-6 

分 类 号：R-33[医药卫生]