水牛胚胎分割技术的研究  

Bisection skills of embryo in buffalo

作　　者：郑海英[1] 杨春艳[1] 李日聪[1] 郑威[1] 张明[2] 梁贤威[1] 牛金涛[3] 

机构地区：[1]中国农业科学院广西水牛研究所,南宁530001 [2]广西大学动物繁殖研究所,南宁530005 [3]济源市畜牧局,河南济源454650

出　　处：《广西农业科学》2010年第2期167-169,共3页Guangxi Agricultural Sciences

基　　金：广西科学研究与技术开发项目(桂科转0626001-3-1;桂科攻0815008-2-4);广西水牛研究所基本科研业务费项目(水牛基0909009)

摘　　要：为探讨水牛桑椹胚去致密化和不同分割方法对水牛切割胚发育效果的影响,将体外受精获得的水牛桑椹胚(第5d)和囊胚(第6～7d)分割后进行体外培养,观察其发育情况。结果显示:(1)水牛桑椹胚经去致密化处理后,其分割成功率(67.69%vs.51.71%)和囊胚发育率(58.30%vs.41.13%)均显著高于未处理组(P<0.01),但囊胚细胞数差异不显著(P>0.05);(2)以直接分割法和单臂分割法分别分割桑椹胚和囊胚,其分割成功率、囊胚发育率和囊胚细胞数三者均无显著差异(P>0.05)。可见,水牛桑椹胚去致密化有利于切割胚的成活和发育,而直接分割法和单臂分割法均可用于水牛桑椹胚和囊胚的分割。The present study was conducted to investigate the effects of eliminating densification and different bisection methods （direct bisection, single arm bisection） of buffalo morula on the development of demi-embryos in buffalo. The buffalo morula （5^th day） and blastula （6-7^th d） produced by IVF were bisected with assistance of micromanipulation system and were cultured in vitro for observation. The results showed that there was significant difference in the successful bisection rate of demi-embryo （67.69% vs. 51.71%） and the developmental rate （58.30% vs. 41.13%） of blastula between eliminating densification and densification treatments of morula （P〈0.01）, while there was no significant difference in cell number of blastula between two treatments （P）0.05）. The successful bisection rate （70.76% vs. 71.49%, 79.02% vs. 80.77%） of demi-embryos and blastula, the developmental rate of blastula （57.27% vs. 57.60%, 67.26% vs. 68.94%） and the average cell number of blastula had no significant difference between two sectioning methods in all treatments （P 〉0.05）. So the eliminating densification of buffalo morula was beneficial to the survival and development of demi-embryo after bisection, and both of two bisection methods could be used for bisecting morula and blastula of buffalo.

关 键 词：水牛 胚胎分割 去致密化 桑椹胚 囊胚 

分 类 号：S823.83[农业科学—畜牧学]