妊娠滋养细胞疾病中ras基因突变及其P^(21)蛋白表达的研究  

Study on ras gene mutation in gastational trophoblastic disease and P 21 protein expression

作　　者：章亚霞[1] 石一复[1] 

机构地区：[1]浙江医科大学附属妇产科医院

出　　处：《中国实验诊断学》1998年第6期296-299,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金

摘　　要：目的了解妊娠滋养细胞疾病分子生物学发病机制中ｒａｓ基因的作用。方法应用ＰＣＲ－ＲＦＬＰ（聚合酶链反应－限制性片段长度多态分析）检测绒毛膜细胞株ＪＡＲ和ＢｅＷｏ、和３６例标本（６例新鲜组织及３０例石蜡标本）中Ｈ－ｒａｓ、Ｋ－ｒａｓ的第１２位点点突变情况；应用免疫组织化学方法检测石蜡标本野生型Ｐ２１和３种突变型Ｐ２１蛋白的表达情况。结果所有被测标本均未见Ｈ－ｒａｓ、Ｋ－ｒａｓ第１２位点的点突变。而野生型Ｐ２１蛋白在妊娠滋养细胞疾病中存在着不同程度的表达，其表达强度依ＧＴＤ恶性程度增加而递减。结论Ｒａｓ基因并不以第１２位点点突变的方式在ＧＴＤ中发挥作用。野生型Ｐ２１参与正常滋养细胞的增殖、分化过程；Objective To inevstigate the role of ras gene in the molecular biogenesis of Gestational Trophoblastic Disease(GTD).Methods The point mutations of H ras and K ras atcodon 12 were detected in 36 GTDs as well as in the JAR,BeWo Choriocarcinoma cell lines by a polymerase chaine reaction restriction fragment length polymorphisms (PCR RFLP);the expression of wild P 21 and three mutant P 21 in formalin fixed ,paraffin emedded tissues was examined using the immunohistohemical method.Results No mutation in K ras,H ras at condon 12 was etected in any of the cases. The wild type p 21 was expressed in the parraffin embedded GTDs,and the expression intensity was decreased according to the increase of malignancy.Conclusion The mutant ras gene at codon 12 does not play an important role in the pathogenesis of GTD.The wild type P 21 may regulate the proliferation and differentiation of the early normal trophoblastic cells,and this function is diminishing according to the increase of malignancy.

关 键 词：妊娠 滋养细胞疾病 RAS基因 点突变 P^21蛋白 

分 类 号：R714.259[医药卫生—妇产科学] R711.740.2[医药卫生—临床医学]