E-钙黏蛋白、β-连环蛋白和Podoplanin在人胃腺癌中的表达及意义  

Expressions of E-cadherin, β-catenin and Podoplanin in Human Gastric Adenocarcinoma and Their Significance

作　　者：夏承东[1] 田怀杲[1] 

机构地区：[1]蚌埠医学院第一附属医院肿瘤外科,安徽蚌埠233000

出　　处：《解剖与临床》2010年第1期17-21,共5页Anatomy and Clinics

摘　　要：目的：探讨E-钙黏蛋白（E—cadhefin）及β-连环蛋白（β-catenin）在人胃腺癌中的表达和在淋巴道转移中的作用。方法：取60例胃腺癌组织样本和30例正常组织，应用免疫组织化学方法观察E—Cad和β-Cat在人胃腺癌组织中的表达，应用Podoplanin标记淋巴管，检测胃腺癌组织中的微淋巴管密度（MLVD）。结果：60例胃腺癌组织中，E—Cad和β—Cat的表达与分化程度、淋巴结转移、TNM分期具有相关性，差异有统计学意义（P〈0．05）。E—cad的表达与肿瘤直径、浸润深度具有相关性，差异有统计学意义（P〈0．05），而β-Cat的异常在这两组中无明显差异（P〉0．05）。Podoplanin只表达淋巴管，癌组织中的LMVD高于正常胃组织（P〈0．01），癌周边缘区的LMVD高于肿瘤中心组织（P〈0．01）。LMVD的表达与分化程度、淋巴结转移、TNM分期具有相关性，差异有统计学意义（P〈0．05）。结论：E—Cad和β—Cat异常表达在胃腺癌进展中起重要作用，在淋巴转移过程中使肿瘤细胞进入淋巴导管起了一定促进作用。Objective：To investigate the expression of E-cadherin and β-catenin in human gastric adenocareinoma and their roles in lymphatic metastasis. Methods：To observe the expression of E-cadherin, β-catenin in 90 samples respectively taken from 60 gastric adenocarcinoma tissues and 30 normal tissues by an immunohistochemical method, and detect the micro-lymphatic vessel density （MLVD） in gastric adenocarcinoma tissues by labeling the lymph vessels with Podoplanin marker. Results：The expression of E-cad and β-cat in 60 gastric adenocareinoma tissues related to the differentiated degree, metastasis through lymph node and TNM staging. The expression of E-cad related to the diameter, infiltrative depth of tumors. The expression of β-cat in gastric adenocarcinoma and normal tissues had not significant difference. Podoplanin was only expressed in lymphatic vessels. The LMVD in gastric adenoearcinoma tissues was higher than in normal gastric tissues,that in marginal zone of tumors was higher than in the center of tumors. The expression of LMVD related to the differentiated degrees, tumour cells transferring through lymph node and TNM staging. Conclusions：The abnormal expressions of E-Cad and β-Cat play important roles in the development of tumors; they contribute to tumor cells into lymphatic ducts.

关 键 词：胃癌 E-钙黏蛋白 Β-连环蛋白 PODOPLANIN 微淋巴管密度 

分 类 号：R735.2[医药卫生—肿瘤]