炎症性肠病患者CD40-CD154共刺激途径的激活及其与内镜下疾病活动程度的相关性研究  

Activation of CD40-CD154 co-stimulatory pathway and its correlation with disease activity in patients with inflammatory bowel disease

作　　者：沈骏[1] 冉志华[1] 郑青[1] 童锦禄[1] 蔡青[1] 朱明明[1] 黄关兰[1] 王天蓉[1] 

机构地区：[1]上海交通大学医学院附属仁济医院上海市消化疾病研究所,200001

出　　处：《中华消化内镜杂志》2010年第2期80-84,共5页Chinese Journal of Digestive Endoscopy

基　　金：上海市科委重点实验室项目（编号：06DZ22027）

摘　　要：目的探讨CD40-CD154共刺激途径在炎症性肠病患者外周循环和肠黏膜中的表达，比较炎症性肠病患者CD40-CD154表达和正常对照者的差异，分析CD40-CD154表达与内镜下疾病活动性的相关性。方法研究对象为克罗恩病患者62例、溃疡性结肠炎患者64例和正常对照者56例。对炎症性肠病患者和正常对照者，分别应用酶联免疫吸附试验（ELISA）、SYBR—green real time PCR方法、免疫组化法，分析血浆中、外周血单个核细胞中、肠黏膜组织中CD40-CD154的表达情况。结果克罗恩病、溃疡性结肠炎患者血浆、外周血单个核细胞及肠黏膜组织中，CD40和CD154的表达均显著高于正常对照者（P均〈0．05），但外周循环和肠黏膜中CD40及CD154的表达和内镜下疾病活动性无关（P均〉0．05）。结论炎症性肠病患者血浆、外周血单个核细胞和肠黏膜中均存在CD40-CD154途径的激活，但CD40-CD154的高表达不能反映内镜下疾病活动程度。Objective To investigate the expression of CD40-CD154 co-stimulatory pathway in peripheral circulation and intestinal mucosa in patients with inflammatory bowel disease （IBD）, the difference between the expression of CD40-CD154 in patients with IBD and that in healthy controls, and the correlation between CD40-CD154 levels and disease activity. Methods A total of 62 patients with Crohn＇s disease （CD）, 64 patients with ulcerative colitis （UC） and 56 healthy controls were enrolled. Enzyme-Linked Immuno Sorbent Assay （ELISA）, SYBR-green real time PCR and immunohistochemical assay were respectively applied to evaluate expression of CD40-CD154 in plasma, mononuclear cells of peripheral blood and intestinal mucosa. Results Levels of CD40 （ P = 0. 000） and CD154 （P = 0. 001 ） in plasma, mononuclear cells of peripheral blood and intestinal mucosa were significantly higher in patients with CD and UC than in healthy controls. However, no correlation between disease activity and CD40-CD154 expression in peripheral circula- tion or intestinal mucosa was detected （ P 〉 0. 05 ）. Conclusion CD40-CD154 pathway activation is found in plasma, peripheral blood mononuclear ceils and intestinal mucosa in patients with IBD, but is not correlated with disease activity.

关 键 词：结肠炎 溃疡性 补体途径 克罗恩病 

分 类 号：R574.6[医药卫生—消化系统]