胃液固有荧光光谱对胃癌诊断价值的初步探讨  

A study on the autofluorescence spectrum of gastric juice in the diagnosis of gastric carcinoma

作　　者：王晶桐[1] 林三仁[1] 周丽雅[1] 王立新[1] 丁世刚 李成刚[1] 耿秋明[1] 赵一鸣[1] 

机构地区：[1]北京医科大学第三附属医院

出　　处：《中华内科杂志》1998年第12期826-828,共3页Chinese Journal of Internal Medicine

摘　　要：目的探讨胃液固有荧光光谱在胃癌诊断中的应用价值。方法胃液高速离心后，应用ＲＦ５０００荧光分光光度计，以２５０ｎｍ紫外光为激发光，测定３００～８００ｎｍ发射光范围内的固有荧光光谱。结果发现胃液荧光光谱有２个峰。胃癌患者与胃内良性病变患者胃液荧光光谱的峰位和峰数基本相同，不同的是胃癌患者第一峰（位于４００ｎｍ处）的相对荧光强度指数（Ｐ１ＦＩ）增大，而第二峰（位于４２０～４３０ｎｍ之间）的相对荧光强度指数（Ｐ２ＦＩ）相对减低。共测定了２４８例各种胃内良、恶性病变患者（其中进展期胃癌３６例）的胃液荧光光谱，计算Ｐ２ＦＩ／Ｐ１ＦＩ，以该比值＜２并且Ｐ１ＦＩ＞２０００为胃癌阳性判断标准，用于进展期胃癌的诊断，其敏感度为８８８９％，特异度为８４９５％。Objective To investigate the value of the autofluorescence spectrum of gastric juice in the diagnosis of gastric carcinoma. Methods The autofluorescence spectrum of gastric juice after being super centrifuged was scanned with RF 5000 spectrophotofluorometer when the excitation wavelength was 250 nm and the emission wavelength ranged from 300 nm to 800 nm. Results The spectra of both benign and malignant gastric diseases were characterized by two peaks. However, the relative fluorescence intensity index of the first peak (P 1FI) at 400 nm of patients with gastric carcinoma was higher than that of patients with benign gastric disease; meanwhile, the fluorescence intensity index of the second peak (P 2FI) between 420～430 nm was relatively low. 248 patients with different gastric diseases including 36 patients with advanced gastric carcinoma were collected in our study. The positive criterion for gastric carcinoma was composed of P 2FI/P 1FI<2 and P 1FI>20.00. The results showed that the sensitivity and specificity of gastric carcinoma were 88.89% and 84.95% respectively. Conclusion The autofluorescence spectrum of gastric juice has a great prospect in the diagnosis of gastric carcinoma.

关 键 词：胃肿瘤 胃液 荧光光谱法 诊断 

分 类 号：R735.204[医药卫生—肿瘤]