脑胶质瘤细胞核DNA含量的流式细胞术分析及意义  

FLOW CYTOMETRIC ANALYSIS OF NUCLEAR DNA GONTENT IN HUMAN GLIOMA CELLS

作　　者：李刚[1] 张锑[1] 李新钢[1] 张庆林[1] 徐从高[1] 

机构地区：[1]山东医科大学附属医院

出　　处：《山东医科大学学报》1998年第4期317-319,共3页Acta Academiae Medicinae Shandong

基　　金：山东省科委科研基金

摘　　要：将手术切除的新鲜脑胶质瘤组织制备成单细胞悬液并进行短期体外培养，采取用流式细胞术测定瘤细胞的ＤＮＡ指数（ＤＩ）、细胞周期分布及细胞分裂增殖指数（ＰＩ）等指标。结果发现，（１）脑胶质瘤Ⅲ、Ⅳ级之间的ＤＮＡ异倍体率差异无显著性，但与其它各组差异有显著性（Ｐ＜０．０５）。（２）对照组的ＤＩ值与脑胶质瘤Ⅰ级差异无显著性，但与其它各组差异有高度显著性（Ｐ＜０．０１）。（３）对照组的Ｓ期与脑胶质瘤Ⅰ、Ⅱ、级之间差异无显著性，但与Ⅲ、Ⅳ级差异有高度显著性（Ｐ＜０．０１）。（４）对照组的ＰＩ值与肿瘤各组差异均有高度显著性（Ｐ＜０．０１）。提示脑胶质瘤细胞核ＤＮＡ的流式细胞术分析能较准确的判定肿瘤的恶性程度，对脑胶质瘤患者的诊断、术后辅助治疗及预后判断均有重要意义。In order to investigate the relationship between changes of nuclear DNA content in human glioma cells (HGC) and clinical patholotical changes of gliomas, 40 fresh isolated glioma specimens were detected with flow cytometry respectively for their DNA index (DI), phases of cell cycle and proliferation index(PI) the results as follows: (1) There was no significant difference between all the incidence of aneuploid in HGC grade Ⅲ and Ⅳ,but there was significant difference between all the other groups.(2) The P value of DI between control group and HGC grade I is higher than 0.05,but the P value is less than 0.01 between all the other gruop. (3) The P value of the percentage of S phase is above 0.05 between control group and HGC grade Ⅰ、Ⅱ,but the P value is less than 0.01 between control group and HGC grade Ⅲ、Ⅳ. (4)There was significant difference between the PI of control group and all the other groups. The analysis of DI, PI and phases of cell cycle is helpful in evaluating the prognosis of glioma patients.

关 键 词：神经胶质瘤 DNA含量 流式细胞术 脑肿瘤 细胞核 

分 类 号：R739.41[医药卫生—肿瘤]