基质金属蛋白酶组织抑制剂-1、2的表达与慢性酒精中毒性肌病的相关性研究  

The correlation between tissue inhibitors of metalloproteinases-- 1 and metalloproteinases- 2 and chronic alcoholic myopathy

作　　者：初海鹰[1] 苗畅贤[2] 王剑锋[2] 

机构地区：[1]大连医科大学组胚教研室,116044 [2]大连市中心医院神经内科

出　　处：《神经疾病与精神卫生》2009年第6期510-512,共3页Journal of Neuroscience and Mental Health

摘　　要：目的研究TIMP-1和TIMP-2的表达与慢性酒精中毒性肌病的相关性。方法通过RT—PCR和Western blotting方法，检测正常大鼠和慢性酒精中毒性大鼠骨骼肌中TIMP-1和TIMP-2的表达差异。结果慢性酒精中毒性大鼠骨骼肌TIMP-1的表达水平增高，腓肠肌更为明显。TIMP-2的表达无显著性差异。结论TIMP-1的增高可能参与了骨骼肌间质纤维增生及其构成的改变，诱导炎性细胞浸润，加重慢性酒精中毒性肌病的发生发展。TIMP-2可能不参与骨骼肌阎质纤维的增生及其构成的改变。Objective To investigae the relationship between the expression of tissue inhibitors of metalloproteinases- 1 （TIMP- 1 ） and metalloproteinases- 2 （TIMP- 2） and chronic alcoholic myopathy （CAM）. Methods The differential expression of TIMP-1 and TIMP-2 between CAM rats and normal rats were determined by RT- PCR and Western blot analysis. Results TIMP- 1 was significantly elevated in CAM rats compared to normal rats, especially in gastroenemius. The TIMP-2 expression, however, showed no difference. Conclusions The over- expression of TIMP- 1 perhaps is involved in the accrementition and reconstitution of mesenchymal fibers of skeletal muscles, and that may facilitate the inflammatory cell infiltration and aggravate the development of CAM. TIMP-2 may not be involved in the accrementition and reconstitution of mesenchymal fibers of skeletal muscles.

关 键 词：慢性酒精中毒性肌病 基质金属蛋白酶组织抑制剂-1 基质金属蛋白酶组织抑制 剂-2 

分 类 号：R746[医药卫生—神经病学与精神病学]