AF-1对内毒素诱导RAW264.7细胞IL-10表达的影响  

Effects of Antiflammin- 1 (AF- 1 ) on expression of IL - 10 of RAW264.7 cells induced by endotoxin

作　　者：张天杰[1,2] 汉建中[1] 刘伟[1] 刘文礼[1] 万静[1] 刘惠君[1] 罗自强[1] 

机构地区：[1]中南大学湘雅医学院生理学系,湖南长沙410078 [2]湘南学院生理学教研室,湖南郴州423000

出　　处：《湘南学院学报（医学版）》2009年第3期5-7,共3页Journal of Xiangnan University（Medical Sciences）

基　　金：国家自然科学基金(30440027;30670770);湖南省重点学科建设资助项目

摘　　要：目的探讨抗炎素-1(Antiflammin-1,AF-1)对脂多糖(LPS)诱导的RAW264.7细胞白细胞介素10(interleukin-10,IL-10)表达的影响。方法采用体外培养巨噬细胞RAW264.7细胞,实验分为正常对照组,LPS组和不同浓度的LPS+AF-1组。应用逆转录PCR(RT-PCR)技术检测IL-10表达的变化。结果 1 μg/ml的LPS刺激RAW264.7细胞后IL-10的表达较正常对照组增加(P<0.05),LPS+AF-1组IL-10表达较LPS组表达显著升高(P<0.05),并具有剂量依赖性。结论 AF-1可促进LPS诱导的RAW264.7细胞IL-10表达的增加。Objective To investigate effects of Antiflammin- 1 （AF- 1 ） on expression of IL- 10 of RAW264.7 cells induced by endotoxin. Methods A macrophage cell line RAW264.7 was employed in an in vitro experiment. LPS and different concentration of AF- 1 were added respectively after RAW264.7 cells were cultured. The interleukin- 10（IL- 10）mRNA levels in supematant was detected by RT- PCR. Results IL- 10 mRNA levels were higher in groups stimulated by 1 μg/ml LPS than those in the blank control group（ P 〈 0.05）. AF- 1 could significantly increased IL - 10 expressions in an concentration - dependently（ P 〈 0.05 and P 〈 0.01 ）. Conclusion AF- 1 could significantly mRNA increased IL- 10 expressions of RAW264.7 cells induced by LPS.

关 键 词：抗炎素-1 白细胞介素10 内毒素 

分 类 号：R392.1[医药卫生—免疫学]