海洋底栖甲藻——利玛原甲藻(Prorocentrum lima)产毒特征的研究  

Studies on Toxin Production of Prorocentrum lima

作　　者：李丽璇[1,2] 汤敬谦[1] 杨维东[1,3] 刘洁生[1,3] 张剑军[1] 李宏业[1,3] 

机构地区：[1]暨南大学生物工程学系,广州510632 [2]中国科学院南海海洋研究所,广州510275 [3]水体富营养化与赤潮防治广东省高等学校重点实验室,广州510632

出　　处：《生态毒理学报》2009年第5期745-750,共6页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金(No.40976065);国家自然科学基金-广东省联合基金重点项目(No.U0733006);广东省自然科学基金(No.9151063201000012)

摘　　要：为了解海洋底栖甲藻——利玛原甲藻(Prorocentrum lima)的产毒特征,阐明腹泻性贝毒(Diarrhetic shellfishpoisoning,DSP)复杂的生物合成机制,采用酶联免疫试剂盒,对利玛原甲藻不同生长期、不同营养盐条件下毒素生成情况进行了研究.结果显示,不同生长期单个利玛原甲藻细胞中大田软海绵酸(Okadaic acid,OA)的含量明显不同,平台期时OA含量最高,与对数中期存在显著差异(p<0.05).低N、P条件下藻的生长状况较差,所能到达的藻密度明显低于对照组(f/2培养基);藻细胞中OA的总含量也低于对照组,但单个藻细胞中OA的含量明显高于对照组(p<0.05).以上结果提示,利玛原甲藻DSP毒素的产生可能与藻所处的环境和藻的生理状态等有关.In order to explore the feature of Diarrhetic shellfish poisoning （DSP）production and elucidate the complicate mechanism of DSP biosynthesis, production of DSP in different growth phases in Prorocentrum lima and under different nutrients conditions was investigated by ELISA. Okadaic acid （OA）concentrations per cell of P. lima were different in various growth phases. The peak of OA was in the stationary phase, which was different significantly to that in the mid - log phase （p 0.05）. P. lima under low concentrations of N, P grew worse than control, while the maximum densities were less than that of control. However, OA concentrations per cell under low concentrations of N, P were higher than that of control. These results suggested that the DSP synthesis in P. lima was related to the environment stress and algal physiological state.

关 键 词：利玛原甲藻 腹泻性贝毒 大田软海绵酸 营养盐 

分 类 号：X171.5[环境科学与工程—环境科学] X172