DNA电化学传感器在DNA损伤研究中的应用  

Application in the Damage of DNA by Electrochemical DNA Sensor

作　　者：孙星炎[1] 徐春[1] 刘盛辉[1] 何品刚[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系

出　　处：《高等学校化学学报》1998年第9期1393-1396,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：利用单链ＤＮＡ分子共价固定在石墨电极表面，采用核酸分子杂交技术，以道诺霉素作为杂交指示剂形成ＤＮＡ电化学传感器．研究了在不同致突变剂的作用下，特定碱基序列的ＤＮＡ在电极表面能否杂交及杂交程度的差异，探讨了ＤＮＡ突变情况及可能的突变机理．Mutation and damage in DNA by interaction, UV irralation, nitrite action and ultrasonication were studied by means of voltammetry using a graphite electrode covalently immobilied with ssDNA 1. Daunomycin was used as a hybidization indicator. When the complementary ssDNA 2 in solution was damaged by phsical or chemical agents, the hybridization of ssDNA 1 and ssDNA 2 was changed. All the results in voltammogram indicated that intercalator cant destroy the double helical structure of DNA, while UV irralation and nitrite action can make the permanent damage. Ultrasonication can reduce polymerization in DNA and the hybridization time was shortened. This may provide a new method for clinical medicine study.

关 键 词：DNA 电化学传感器 道诺霉素 杂交 突变 损伤 

分 类 号：Q523.03[生物学—生物化学] O657.1[理学—分析化学]