液相色谱-质谱联用分析动物内脏和肌肉组织中的全氟化合物  

High Performance Liquid Chromatography-Tandem Mass Spectrometry for Determination of Perfluorinated Compounds in Animal Visceral and Muscle Tissues

作　　者：王杰明[1] 王丽[2] 冯玉静[2] 潘媛媛[1] 史亚利[1] 蔡亚岐[1] 

机构地区：[1]中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室,北京100085 [2]中国农业大学生物学院,北京100193

出　　处：《食品科学》2010年第4期127-131,共5页Food Science

基　　金：国家高技术研究计划项目(2007AA06Z405);中国科学院重要方向性项目(KZCX2-YW-420-1);国家自然科学基金创新群体项目(20621703)

摘　　要：建立液相色谱-质谱联用检测动物内脏(猪心、猪肝、猪肾、鸡心和鸡肝)和肌肉(猪里脊肉和鸡胸肉)组织中11种全氟化合物(PFCs)的分析方法。11种PFCs包括9种常见PFCs和2种调聚酸。首先考察对比了WAX和MAX两种固相萃取小柱对11种PFCs的回收情况,最终选用WAX柱对样品的萃取液进行净化。通过样品前处理方法的比较和优化,对动物内脏和肌肉组织分别采用离子对液液萃取和碱消解法。最后分析检测了农贸市场上的猪肝等多种实际样品,除了PFTA和2种调聚酸回收率较低外,其他8种PFCs的实际样品的加标回收都在80%～120%之间,方法检出限为0.002～0.032ng/g。A high performance liquid chromatography-electrospray ionization-tandem mass spectrometric （HPLC-ESI-MS/MS） method was proposed for the determination of 11 perfluorinated compounds （PFCs） including 9 common PFCs,2-perfluorooctyl ethanoic acid （FOEA） and 2H-Perfluoro-2-decenoic acid （FOUEA） in animal visceral （pork heart,pork liver,pork kidney,chicken heart and chicken liver） and muscle （pork tenderloin and chicken breast） tissues.WAX and MAX SPE cartridges were compared for the difference in recovery of 11 PFCs and WAX SPE cartridge was selected.Ion-pair liquid-liquid extraction and alkaline digestion were determined to be optimal methods for sample pretreatment of visceral and muscle tissues,respectively.In the determinations of real samples including pork liver,etc purchased from a local farmers market,the developed method exhibited low spike recoveries of PFTA,FOEA and FOUEA,while the spike recoveries of other 8 PFCs were found to be in the range of 80%-120%.The limit of detection of the method ranged from 0.002 to 0.032 ng/g.

关 键 词：全氟化合物 内脏 肌肉 液相色谱-质谱联用 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]