厌氧发酵产氢细菌的分离和鉴定及产氢特性  

Isolation,identification and H_2-producing characterization of anaerobic fermentation strain

作　　者：马晓轩[1,2] 李珊珊[1,2] 范代娣[1,2] 陈火晴[1,2] 朱晨辉[1,2] 骆艳娥[1,2] 米钰[1,2] 

机构地区：[1]西北大学化工学院陕西省可降解生物医用材料重点实验室,陕西西安710069 [2]西北大学化工学院陕西省生物材料与发酵工程技术研究中心,陕西西安710069

出　　处：《化学工程》2010年第3期68-71,共4页Chemical Engineering(China)

基　　金：陕西省自然科学基金(SJ08B03);教育部高等学校博士学科点专项科研基金(20096101120023)

摘　　要：获得优良的产氢菌株,鉴定并研究其产氢特性,为提高其产氢能力提供基础。对样品进行预处理和葡萄糖梯度驯化处理后,采用厌氧培养技术分离单菌落,进行菌株形态学观察、生理生化试验及16S rDNA分子生物学鉴定,并采用分批式厌氧发酵研究其产氢特性。结果表明:其中产氢最优的菌株FML-C1为肠杆菌属细菌,葡萄糖为其产氢的最优碳源,氮源则以酵母粉和蛋白胨混合最佳,最适生长和产氢温度为35℃,最佳初始pH值为6.0,该条件下菌株的气相末端产物中氢体积分数达56.51%。菌株FML-C1为一株良好的厌氧发酵产氢菌,可进一步优化其产氢工艺,以提高其产氢能力。In order to improve the H2 production capacity, it is necessary to select the good H2-producing strain and research its characteristics. After pretreatment and gradient-glucose acclimation, the anaerobic culture techniques were adopted for hydrogen production bacteria screening, the analysis of morphological characteristics, physiological biochemical tests and 16S rDNA sequencing. According to the experimental results, Enterobacter FML-C1 is identified as the best strain for H2-producing. Glucose and the mixture of yeast and peptone are the best carbon and nitrogen sources for H2-production, respectively. The optimal temperature is 35 ℃ and initial pH value is 6.0. The H2 volume fraction is up to 56.51% of the total gas. Enterobacter FML-C1 is a good stain for biohydrogen production. It is worthy of further investigation for improving its H2 production capacity.

关 键 词：生物制氢 菌种筛选 分离鉴定 16S RDNA 厌氧发酵 

分 类 号：TQ116.2[化学工程—无机化工]