裙带菜多糖3种脱蛋白质方法的比较研究  

Comparative Study on Three Methods of Removing Proteins from Polysaccharide Extract from Undaria pinnatifi da

作　　者：葛炳艳[1,2,3] 杨波 

机构地区：[1]国家教育部抗肿瘤天然药物工程研究中心 [2]哈尔滨商业大学生命科学与环境科学研究中心 [3]哈尔滨商业大学博士后工作站,黑龙江哈尔滨150076

出　　处：《食品与药品》2010年第3期96-98,共3页Food and Drug

摘　　要：目的筛选裙带菜多糖脱除蛋白质的最佳方法。方法采用Sevag法、三氯乙酸法和酶法对裙带菜多糖进行脱蛋白质处理,比较不同方法处理后多糖的脱蛋白质率及多糖损失率,确定最佳的除蛋白质方法。结果酶法的多糖损失率最低,为10.22%,脱蛋白质率最高,为61.10%;三氯乙酸法多糖损失率12.13%,脱蛋白质率57.37%;Sevag法多糖损失率14.21%,脱蛋白质率54.12%。结论酶法是裙带菜多糖脱蛋白质的最佳方法。Objective To remove the proteins from the polysaccharide extract from Undaria pinnatifida and select the best method of removing proteins.Methods Three different methods of removing proteins including the Sevag method,trichloroacetic acid（TCA） method and enzymic method were compared by analyzing the percentages of deproteinization and lost polysaccharide.The optimum method of removing proteins was selected.Results The enzymic method had a lower percentage of lost polysaccharide,which was 10.22 %,but a high percentage of deproteinization,which was 61.10 %.For TCA,the percentage of deproteinization was 57.37 %,the percentage of lost polysaccharide was 12.13 %.The percentages of deproteinization and lost polysaccharide of Sevag method were 54.12 % and 14.21 %,respectively.Conclusion The enzymic method can be the best method of removing proteins from the polysaccharide extract from Undaria pinnatifida.

关 键 词：裙带菜多糖 脱蛋白质 酶法 

分 类 号：Q946.3[生物学—植物学]