RP-HPLC双波长梯度洗脱法同时测定通塞脉微丸中阿魏酸、甘草素、肉桂酸、哈巴俄苷的含量  

Determination of Ferulic Acid,Liquiritigenin,Cinnamic acid and Harpagoside in Tong SaiMai Tinypil by HPLC

作　　者：陶金华[1] 狄留庆[1] 文红梅[1] 赵晓莉[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210029

出　　处：《现代中药研究与实践》2010年第2期66-69,共4页Research and Practice on Chinese Medicines

基　　金：国家自然基金项目(30873401);国家科技部863计划(2003AA2Z3247);江苏省自然基金项目(BK2005150);江苏省"青蓝工程"科技创新团队支持计划

摘　　要：目的建立以RP-HPLC双波长法同时测定通塞脉微丸中阿魏酸、甘草素、肉桂酸、哈巴俄苷4个有效成分含量的方法。方法采用Packing Material HederaODS-2柱(250 mm×4.6 mm,5μm)柱,以乙腈-1.0%磷酸水为流动相,梯度洗脱;双波长检测,λ_1=320 nm,λ_2=276 nm;流速为1.0 mL·min^(-1);柱温30℃;结果阿魏酸、甘草素、肉桂酸和哈巴俄苷分别在32.04～160.2、12.2～61.0、12.24～61.2、11.88～59.4μg·mL^(-1)内有良好线性关系,r≥0.9995。4个份均能达到基线分离,各组分的平均回收率在97.30%～100.94%之间。结论本方法简便,准确,重现性好,可作为该制剂质量控制的方法。Objective To establish a HPLC method with gradient elution for the determination of ferulic acid, liquiritigenin, cinnamic acid and harpagoside in Tong SaiMai Tinypil. Methods The separation was performed on a Packing Material HederaODS-2 column（250 mm×4.6 um,5 um） with the mobile phase consisting of acetomitrile-1% phosphoric acid for gradient elution, ferulic acid, liquiritigenin, cinnamic acid and harpagoside were determined by dual wave length of 320 nm（λ1） and 276 nm（λ2）, with flow velocity 1.0 mLomin1 and column temperature at 30℃. Results The linearities were in the ranges of 32.04-160.2ug·mL^-1 for ferulic acid, 12.2-61.0ug·mL^-1 for liquiritigenin,＇and 12.24-61.2ug·mL^-1 for cinnamic acid, and 11.88-59.4ug·mL^-1 for harpagoside, respectively. Conclusion This method is convenient, accurate and exceptional reproducibility. It can be used for quality control in production of Tong SaiMai tinypill.

关 键 词：RP-HPLC 通塞脉微丸 甘草素 阿魏酸 肉桂酸 哈巴俄苷 

分 类 号：R927.2[医药卫生—药学]