大鼠局灶性脑缺血/再灌注后血脑屏障通透性改变时相的研究  

Time-phase study on the permeable changes of blood brain barrier after focal cerebral ischemia/reperfusion(I/R) in rats

作　　者：杨莹[1] 史秀丽[1] 何玮[1] 傅佳[1] 

机构地区：[1]安徽医科大学第一附属医院神经内科,安徽合肥230022

出　　处：《中风与神经疾病杂志》2010年第2期125-127,共3页Journal of Apoplexy and Nervous Diseases

基　　金：安徽省教育厅自然科学基金(No.2005KJ250)

摘　　要：目的探讨大鼠局灶性脑缺血/再灌注(I/R)后氧自由基(OFR)与血脑屏障(BBB)通透性改变的时相关系。方法成年健康SD大鼠随机分为脑缺血/再灌注组、正常组及假手术组,分别在脑缺血2h后再灌注各时间点观察脑组织EB含量,血清超氧化物酶(SOD)活力、丙二醛(MDA)含量以及脑组织基质金属蛋白酶2(MMP-2)的表达。结果OFR与脑组织EB含量有相同时相变化趋势,参与BBB通透性增高的整个过程,同期伴随MMP-2表达,且OFR峰值期与BBB通透性增高的第一高峰时相符合。结论OFR是导致BBB通透性增高的重要因素,尤其在其通透性增高的早期发挥重要作用,MMP-2激活可能是OFR损伤BBB的机制之一。Objective The time-phase relationship between oxygen free radical（OFR） and the blood brain barrier（BBB） permeability was observed.Methods Healthy SD rats were randomly divided into the control group,the sham group and the I/R group.The activity of SOD,the content of MDA and the expression of MMP-2 at each time point of reperfusion after 2h cerebral ischemia were observed.Results The content of OFR and EB had the same time-phase variability,while the expression of MMP-2 happened simultaneously.What＇s more,the mode of OFR coincided with the first mode of EB.Conclusion OFR participated in the whole procedure of the increased BBB permeability,especially the first mode of the increased BBB permeability.The activated MMP-2 may be one of the mechanisms of the BBB injury caused by OFR.

关 键 词：脑缺血再灌注损伤 血脑屏障 氧自由基 基质金属蛋白酶2 

分 类 号：R743.3[医药卫生—神经病学与精神病学]