高血压脑出血早期血肿周围脑组织细胞凋亡与凝血酶表达相关性的研究  

Association between cell apoptosis and the expression of thrombin in perihematoma tissue in early hypertensive intracerebral hemorrhage

作　　者：彭绍鹏[1] 任海军[1] 高俊玮[1] 侯博儒[1] 张建生[1] 霍龙伟[1] 

机构地区：[1]兰州大学第二附属医院神经外科,甘肃兰州730030

出　　处：《中风与神经疾病杂志》2010年第2期146-148,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨高血压脑出血（HICH）早期血肿周围脑组织细胞凋亡与凝血酶表达的关系。方法取36例高血压脑出血患者血肿周围脑组织作为实验组,按发病至手术的时间分为5组：〈6h（7例）、6-12h（11例）、12-24h（8例）、24-48h（6例）、48-72h（4例）。另将侧脑室肿瘤患者经皮层入路取得的正常脑组织作为对照组。采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（TUNEL）检测神经细胞凋亡率,免疫组织化学方法检测凝血酶表达。结果对照组脑组织基本无TUNEL阳性细胞（0.32±0.037）;〈6h组有轻微表达（7.19±2.53,P〈0.05）,发病6h后逐渐增高（15.11±3.69,P〈0.01）,12-24h增高显著（28.26±7.83,P〈0.01）,并于24-48h达高峰（53.79±6.35,P〈0.01）,48-72h组有所回落,但仍维持较高水平（38.23±3.29,P〈0.01）。凝血酶表达变化与此一致。相关分析显示：细胞凋亡与凝血酶表达呈显著正相关（r=0.7451,P〈0.05或P〈0.01）。结论H ICH患者血肿周围脑组织中存在细胞凋亡。Objective To investigate the relationship between cell apoptosis and expression of thrombin in the perihematoma tissue in early hypertensive intracerebral hemorrhage（HICH）.Methods 36 samples were obtained from the perihematoma region of HICH patients and were divided into 5 groups according to the time from onset to operation： 6h group（n=7）,6-12h group（n=11）,12-24h group（n=8）,24-48h group（n=6） and 48-72h group（n=4）.The normal control group were collected from the patients with lateral ventricle tumor who were operated by transcortical approach.The terminal deoxynucleotidyl-transferase mediated dUTP-biotin nick end labeling（TUNEL）method was used to detect apoptosis and the expression of thrombin was observed by immunohistochemistry.Results The TUNEL positive cells were almost not found in the control group（0.32±0.037） and it had a slight expression in 6h group（7.19±2.53,P 0.05）.It increased gradually 6h after the onset of HICH（15.11±3.69,P 0.01） and reached the maximum during 24-48h（53.79±6.35,P 0.01）.48-72h group showed slightdrop,but still maintain at a high level（38.23±3.29,P〈0.01）.The expression of the thrombin was consistent with the apoptosis.The correlation analysis showed that the TUNEL positive cells was positively correlated to the expression of the thrombin（P〈0.05 or P〈0.01）.Conclusion Apoptosis cells exist in perihematomal region in hypertensive intracerebral hemorrhage.The expression of thrombin is a critical step in apoptotic cell death after hypertensive intracerebral hemorrhage.

关 键 词：高血压脑出血 细胞凋亡 凝血酶 

分 类 号：R743.34[医药卫生—神经病学与精神病学]