慢性酒精中毒性肌病线粒体损伤的实验研究  

The study of mtDNA lesions in the chronic alcoholic myopathy

作　　者：王剑锋[1] 齐珊珊[1] 王永平[2] 钟丽珍[1] 张利[1] 初海鹰[3] 

机构地区：[1]大连市中心医院神经内二科,辽宁大连116033 [2]中国医科大学临床医学系,2008级9班辽宁沈阳110000 [3]大连医科大学组胚教研室,辽宁大连116044

出　　处：《中风与神经疾病杂志》2010年第2期149-152,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的研究在慢性酒精中毒性肌病中的线粒体损伤情况并探讨受损原因。方法建立大鼠慢性酒精中毒性肌病模型,采用分光光度计法、Western Blot和流式细胞仪检测实验组大鼠骨骼肌各抗氧化指标(SOD、GSH-Px、MDA)、线粒体膜蛋白细胞色素C、线粒体膜流动性和线粒体膜电位的变化。结果实验组大鼠骨骼肌中MDA含量升高,SOD、GSH-Px活性降低,细胞色素C的表达减少,线粒体膜流动性下降,线粒体膜电位降低。结论慢性酒精中毒性肌病中线粒体发生损伤,提示与活性氧(ROS)增多有关。Objective To study mtDNA lesions in the chronic alcoholic myopathy and to explore the reason of the lesions.Methods Spectrophotometer,western blotting and flow cytometry analysis were utilized to determine the SOD、GSH-Px and MDA,the expression of cyt-C,mitochondrial membrane fluidity and mitochondrial membrane potential（△Ψm） in the experimental group.Results MDA were significantly higher,activity of SOD and GSH were lower,mitochondrial membrane fluidity were lower,the expression of cyt-C decrease and mitochondrial membrane potential were lower in the experimental group.Conclusion The mtDNA lesions in the CAM relates to the increase of ROS.

关 键 词：慢性酒精中毒性肌病 线粒体膜流动性 线粒体膜电位 细胞色素C 

分 类 号：R741[医药卫生—神经病学与精神病学]