Nogo-A及其受体与癫痫大鼠海马苔藓纤维发芽的关系  

The relationship of Nogo-A and NgR with the mossy fiber sprouting in hippocampus of epileptic rat

作　　者：杨雷 赵世刚[2] 刘罡[2] 冯冠青[2] 

机构地区：[1]内蒙古医院神经内科,内蒙古呼和浩特010017 [2]内蒙古医学院第一附属医院神经内科,内蒙古呼和浩特010050

出　　处：《中风与神经疾病杂志》2010年第2期160-163,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的研究神经轴突再生抑制因子Nogo-A及其受体NgR蛋白在癫痫大鼠海马细胞内的表达水平,探讨Nogo-A及NgR与癫痫大鼠海马苔藓纤维发芽(mossy fiber sprouting,MFS)发生、发展的关系,为进一步明确癫痫发病机制提供研究依据。方法采用红藻氨酸(KA)颈部皮下注射法建立大鼠癫痫模型。(1)采用链霉素抗生物素蛋白-过氧化物酶(SP)法对大鼠海马中Nogo-A及NgR蛋白进行检测,并用图像分析仪对染色结果进行分析;(2)应用Timm's硫化银法对大鼠海马脑片进行染色,并对结果进行半定量分析。结果(1)实验组Nogo-A蛋白表达与对照组差别无显著性意义(P>0.05);(2)注射KA后4h NgR蛋白表达降低,至造模后7d,NgR蛋白表达升高至正常水平,实验组与对照组之间比较,组内、组间表达均有显著性差异(P<0.01);(3)Timm's硫化银染色结果显示:最早在造模2w后可见海马苔藓状纤维发芽,染色结果为淡染,至造模后2个月发芽脑片染色为深染。结论红藻氨酸造模后癫痫大鼠海马Nogo-A蛋白表达无改变;NgR蛋白表达在造模初期呈现"降量调节";癫痫大鼠海马苔藓状纤维发芽的发生可能与NgR蛋白水平表达下调有关。Objective To study the expression level of the regeneration inhibiting factor of neural axon Nogo-A and NgR proteins in hippocampus of epileptic rat,and explore the relationship of Nogo-A and NgR with mossy fiber sprouting（MFS）,then to provide gist for the research of the outbreak of epilepsy.Methods Establishing animal model of temporal lobe epilepsy by injecting KA subcutaneously.（1） Streptavidin-peroxidose（s-p） was performed to detect the expression level of Nogo-A and NgR proteins and then the pigmentation was analyzed with image analyzer.（2） The hippocampus of epileptic rat was dyed with Timm＇s stain and its result was analyzed semi-quantitatively.Results（1） No significant difference was found among Nogo-A groups and normal groups（P﹥0.05）.（2） The expression level of NgR protein decreased in the group of four hours and then returned to the normal level in the group of seven days after KA injecting.There were significant differences both in and between the NgR groups and normal groups.（3）The results of Timm＇s stainshowed a light color after two weeks and then in dark after two months.Conclusions The expression level of Nogo-A protein in hippocampus of epileptic rat remained unchanged and that of NgR protein showed down-regulation at the early stage after KA injecting.It can be drawn that the occurrence of mossy fiber sprouting is related to the down-regulation of NgR protein.

关 键 词：苔藓纤维发芽 NOGO-A NGR 免疫组化 

分 类 号：R742.1[医药卫生—神经病学与精神病学]