青蒿琥酯和TRAIL对前列腺癌细胞凋亡诱导作用的实验研究  

Experiment research on inducing apoptosis in prostate cancer cell with artesunate and TRAIL

作　　者：周天贵[1] 周承贵 袁红纲[1] 陈先国[3] 袁丁[4] 

机构地区：[1]三峡大学第一临床医学院泌尿外科,湖北宜昌443003 [2]湖北省枝江市安福寺镇中心卫生院外科,443211 [3]华中科技大学同济医学院同济医院泌尿外科,武汉430030 [4]三峡大学医学院,湖北宜昌443003

出　　处：《重庆医学》2010年第6期663-665,共3页Chongqing medicine

摘　　要：目的探讨青蒿琥酯和肿瘤坏死因子相关的凋亡诱导配体(TRAIL)对前列腺癌细胞(PC-3、LNCaP)凋亡的诱导作用。方法培养前列腺癌PC-3、LNCaP细胞,分别加入不同浓度的青蒿琥酯和TRAIL,随机分为7个组:对照组、青蒿琥酯Ⅰ组(低剂量组)、青蒿琥酯Ⅱ组(高剂量组)、TRAILⅠ组(低剂量组)、TRAILⅡ组(高剂量组)、TRAIL(1 ng/mL)加青蒿琥酯(1mmol/L)Ⅰ组及TRAIL(1 ng/mL)加青蒿琥酯(5 mmol/L)Ⅱ组。流式细胞仪检测青蒿琥酯和TRAIL诱导前列腺癌细胞凋亡的凋亡率。结果青蒿琥酯和TRAIL均可增加前列腺癌细胞凋亡的凋亡率,二者联合使用诱导的前列腺癌细胞的凋亡率明显高于两种药物单独使用。结论青蒿琥酯与TRAIL联合使用对前列腺癌存在诱导凋亡的协同作用。青蒿琥酯能诱导提高前列腺癌细胞对TRAIL的敏感性,加强TRAIL的诱导肿瘤细胞凋亡的作用。Objective To investigate the effects of artesunate on prostate cancer cell lines PC-3 and LNCaP in vitro. Methods Prostate cancer cell lines PC3 and LNCaP were cultured and divided into seven groups： normal control group, artesunate I group （low doses）,artesunate II group （high doses）,TRAII. I group （low doses）,TRAIl. II group （high doses）,artesunate plus TRAIL group I,artesunate plus TRAIl. group II. Flow eytometry（FCM）was used to examine the apoptosis rate in prostate cancer cell in- duced by artesunate and TRAIl.. Results Both artesunate and TRAIl. could increase the apoptosis rate in prostate cancer cell,the apoptosis rate using artesunale associated with TRAIL was much higher than that using artesunate or TRAIL alone signifieantly（P d0.01）. Conclusion There may be synergistic effect on inducing apoptosis in prostate cancer cell with artesunate and TRAIl.. Ar tesunate can increase the sensitivity of prostate cancer cell to TRAIL,and strengthen the apoptosis induced by TRAIl. in prostate cancer cell. Artesunate may be a new preparation for anti-prostate cancer cell with curative effect and little side effect,it may have favourable market prospect.

关 键 词：青蒿琥酯 肿瘤坏死因子相关的凋亡诱导配体 前列腺癌 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]