不同氮素形态及比例对烤烟多酚及相关酶活性动态的影响  

Effects of Different NO_3^--N/NH_4^+-N Ratios on Polyphenols and Related Enzyme Activity of Flue-cured Tobacco Leaves

作　　者：孟祥东[1] 赵铭钦[1] 李元实[2] 孙国伟[2] 韩富根[1] 

机构地区：[1]河南农业大学烟草学院/国家烟草栽培生理生化研究基地,河南郑州450002 [2]吉林烟草工业有限责任公司,吉林延吉133001

出　　处：《江西农业大学学报》2010年第1期25-30,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：吉林省烟草工业有限责任公司重大科技攻关项目(JY2006012)

摘　　要：以烤烟品种吉烟9号(Nicotianatobacum L.)为试验材料,通过盆栽试验,研究不同氮素形态对烤烟多酚及相关酶活性动态的影响。结果表明,氮素形态对烤烟多酚及酶活性有明显影响,移栽30d后,50%硝态氮+50%铵态氮处理多酚类物质含量明显提高。在移栽后30～75d,50%硝态氮+50%铵态氮处理POD和PAL一直保持相对较高水平,且PAL活性随着硝态氮比例的增加而增强,栽后75d达到高峰后迅速降低;在移栽后60d,75%铵态氮+25%硝态氮处理PPO活性达到峰值;生长前期APX活性随着铵态氮比例的增加而增强,移栽后75d达到峰值而后迅速降低且以50%硝态氮+50%铵态氮处理活性最强。A study was conducted on the effect of different nitrogen forms on polyphenols and related enzyme activity in flue-cured tobacco leaves.The nitrogen forms had obviously influence on polyphenols and related enzyme activity of flue-cured tobacco,the content of the chlorogenic acid,rutin and total phenols increased 30 d after transplanting,the content of polyphenols of 50% ammonium nitrogen ＋ 50% nitrate nitrogen treatment increased obviously.POD and PAL activity of 50% ammonium nitrogen ＋50% nitrate nitrogen treatment was the highest 30-75 d after transplanting,and the activity of PAL increased by increasing nitrate nitrogen and peaked 75 d after transplanting then decreased quickly.PPO activity of 75% ammonium nitrogen ＋25% nitrate nitrogen treatment reached the peak on the 60 d after transplanting.The activity of APX increased by increasing ammonium nitrogen during the early stages of growth,and peaked on the 75 d after transplanting and then decreased quickly and the activity of APX of 50% ammonium nitrogen ＋ 50% nitrate nitrogen treat-ment was the strongest.

关 键 词：烤烟 氮素形态 多酚 酶活性 

分 类 号：S572.01[农业科学—烟草工业]