芦荟皮叶绿素的提取及稳定性研究  

Extraction and Stability of Chlorophyll from the Husk of Aloe vera

作　　者：陈新香[1] 蔡碧琼[1] 蔡珠玉[1] 陈杰博[1] 苏金为[1] 

机构地区：[1]福建农林大学生命科学学院,福建福州350002

出　　处：《江西农业大学学报》2010年第1期175-180,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：福建省自然科学基金(B0710008);福建农林大学青年教师科研基金项目(06A11)

摘　　要：以芦荟皮为原料,采用醇提法提取芦荟皮中的叶绿素。通过单因素试验分析了浸提溶剂种类、浸提溶剂浓度、浸提温度、浸提时间和料液比等5个主要因素对芦荟皮叶绿素提取效果的影响。在单因素的基础上,采用L9(34)正交试验设计优化芦荟皮叶绿素的提取工艺,并对叶绿素的光稳定性、热稳定性以及耐酸碱性等方面进行研究。结果表明:体积分数φ(乙醇)=85%、浸提温度80℃、浸提时间5.0h、料液比1∶50是最优工艺条件,叶绿素的提取率可达0.1970mg/g。芦荟皮叶绿素具有良好的耐热性;自然光长时间照射会影响叶绿素的稳定性;在弱碱性介质环境中,叶绿素稳定性良好。The husk of Aloe vera was used as the raw material for extracting chlorophyll with ethanol as extractant.Using single factor analysis method,the influences of extracting solvents,extracting solvent concentration,extraction temperature,extraction time and ratio of liquid to solid on extraction efficiency were studied.L9（3^4） orthogonal experiment was adopted for getting the optimal mixed technique parameters.Moreover,the photo-stability and thermo-stability of the chlorophyll and its tolerance for acidification were further studied.The result indicated that the extraction ratio of chlorophyll could amount to 0.1970 mg/g with 85% of ethanol,80 ℃ of extraction temperature,5.0 h of extraction time and 1：50 of liquid to solid ratio.The study also showed that chlorophyll had good tolerance for thermo-stability.The stability of the chloro-phyll could be influenced by lightening.Chlorophyll was relatively stable under alkalescent circumstances.

关 键 词：芦荟皮 叶绿素 提取 稳定性 

分 类 号：S567.239[农业科学—中草药栽培]