豫麦34低分子量谷蛋白亚基一个新基因的克隆  

Molecular Cloning of a Novel Low-molecular-weight Glutenin Subunit Gene from Yumai 34

作　　者：张丽[1] 李玉阁[1] 苏亚蕊[1] 张大乐[1] 黄世全[1] 李锁平[1] 

机构地区：[1]河南大学农业生物技术研究所,河南开封475001

出　　处：《麦类作物学报》2010年第2期210-214,共5页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(30971774)

摘　　要：为了解强筋优质小麦品种豫麦34的低分子量谷蛋白亚基(LMW-GS)的基因构成,利用普通小麦LMW-GS基因特异引物,采用PCR扩增技术从中克隆得到一个LMW-GS新基因LMWY34(GenBank No.GU183486)。该基因具有LMW-GS基因的典型结构特征,编码区全长906 bp,编码302个氨基酸。推导氨基酸序列显示,LMWY34的编码产物N-端具有LMW-m型低分子量谷蛋白亚基的典型特征,与已报道的GluD3-4位点编码的LMW-GS基因序列有很高的一致性(98.68%)。In the present paper,a novel low-molecular-weight glutenin subunit（LMW-GS）gene,LMWY34（GenBank No.GU183486）,was amplified and cloned with a pair of specific primers of LMW-GS genes from the wheat variety Yumai 34.LMWY34 was a typical LMW-GS gene with whole coding region of 906 bp,which encoding a protein of 302 amino acid residues.Comparisons of amino acid sequences showed that it had the typical structure of LMW-m type in N-terminal region.It had high sequence similarity with the reported LMW-GS genes encoded by the GluD3-4 locus.

关 键 词：小麦 低分子量谷蛋白亚基 基因克隆 序列分析 

分 类 号：S512.1[农业科学—作物学] S326