黑龙江省完达山东部林区马鹿微卫星位点的筛选  

Selection of Microsatellite loci in Wapiti from Eastern Region of Wanda Mountains

作　　者：张辉[1] 张明海[1] 罗理扬[1] 

机构地区：[1]东北林业大学野生动物资源学院,哈尔滨150040

出　　处：《野生动物》2010年第2期59-62,68,共5页

基　　金：国家自然科学基金资助项目(30870309);黑龙江省自然科学基金重点项目(ZJN-0501)

摘　　要：利用黑尾鹿、白尾鹿等近缘物种的18对微卫星引物,对2个东北马鹿肌肉DNA样品和176个粪便DNA样品进行扩增,通过毛细管电泳法筛选适合东北马鹿的微卫星位点。结果表明,其中3个位点为高度多态性位点(PIC>0.5),1个为中度多态性位点(0.5>PIC>0.25)。共检测到26个等位基因片段,平均每个位点6.5个等位基因。多态信息含量0.393～0.827,个体识别率0.643～0.958,累积个体识别率为0.9999,累积非父排除率为0.9976。这4个多态性微卫星位点为东北马鹿的遗传研究提供了理论依据。18 pairs of DNA primers of microsatellites from black-tailed and white tailed deer were used to amplify DNA microsatellite of muscle of 2 wapiti（Cervus elaphus xanthopygus） and 176 samples of feces of wapiti.Amplified products of 3 pairs of 18 pairs of primers were highly polymorphic in capillary electrophoresis and 1 was medium polymorphic.26 allelic loci were tested,with 6.5 alleles per locus,polymorphic range 0.393 to 0.827,discrimination range 0.643 to 0.958,total power of discrimination 0.9999 and the probability of paternity exclusion was 0.9976.The above 4 loci were capable of applying to genetic research of wapiti.

关 键 词：马鹿 微卫星位点 粪便DNA 

分 类 号：Q75[生物学—分子生物学] Q959.842