异体脂肪源干细胞移植对大鼠的抗衰老作用  

Anti-aging effect of allografting adipose-derived stem cells in rats

作　　者：杨春[1] 李东飞[1] 戴景兴[1] 杨会营[1] 余美春[1] 陶晖[1] 余磊[1] 原林[1] 

机构地区：[1]南方医科大学人体解剖学教研室,广州510515

出　　处：《解剖学报》2010年第1期87-92,共6页Acta Anatomica Sinica

基　　金：国家重点基础研究计划(973计划)项目(2007CB512705);国家自然科学基金青年面上项目(30801464)资助

摘　　要：目的从筋膜学的角度设计观察脂肪源干细胞(ADSCs)异体移植对衰老模型大鼠体内自由基和免疫功能的影响,探索一种新的抗衰老方法。方法30只SD大鼠随机分成空白对照组(A组)、模型组(B组)和治疗组(C组)。B、C组大鼠注射D-半乳糖(D-gal)复制亚急性衰老模型。C组大鼠在造模8周后,经尾静脉输入体外扩增培养的脂肪源干细胞3×106个。治疗2周后,检测各组大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、血清白细胞介素-2(IL-2)水平和脾脏指数。结果与空白对照组比较,模型组大鼠血清SOD、NO、IL-2水平及脾脏指数均显著性降低,血清MDA水平显著升高;移植ADSCs后,治疗组大鼠较模型组大鼠血清SOD、NO、IL-2水平及脾脏指数均有所提高,而MDA含量显著降低。结论异体移植ADSCs可有效增强大鼠机体清除自由基和抗氧化能力,提高机体免疫功能,从而延缓D-gal诱发的大鼠衰老。Objective To observe the effect of transplanting the adipose-dervived stem cells （ADSCs） on free radical metabolism and immune function of rat aging model induced by D-galactose from fasiaology perspective; to explore a new method for anti-aging. Methods The ADSCs were cultured in vitro. Thirty male SD rats were randomly divided into 3 groups： normal control group（A） , aging model group（B） and treat group（C）. Ten rats in each group. Rats in B and C groups were injected D-galactose continually into make the sub-acute aging model rats. After 8-week injections of D- galactose, rats in group C were injected ADSCs（3 × 10^6/ml） through caudal vein. After 2-week transplantions of ADSCs, T-SOD, CuZn-SOD, MDA, NO, IL-2 and spleen index levels in serums of each group were detected and compared among the three groups. Results Compared with the A group, the SOD, NO, IL-2 level and spleen index in serum in group B decreased significantly, while the contents of MDA increased significantly. Compared with group B, the SOD, NO, IL-2 level and spleen index in serum in group C had been improved, and the contents of MDA decreased significantly. Conclusion Transplanting ADSCs can improve the antioxidant ability and strengthen the cellular immune function of aging rats. Further more, it can delay the ageing procedure induced by D-galaetose in rats.

关 键 词：脂肪源干细胞 抗衰老 移植 筋膜学 大鼠 

分 类 号：R394.2[医药卫生—医学遗传学]