过氧化物酶体增殖因子激活受体γ、环氧合酶-2在子宫内膜癌中的表达及超微结构定位  

Expression and ultrastructure location of PPAR-γ and COX-2 in endometrial carcinoma

作　　者：卞海燕[1] 李媛媛 吴洪娟 刘雨清[1] 

机构地区：[1]潍坊医学院病理学教研室,山东潍坊 [2]形态学实验室,山东潍坊261042

出　　处：《解剖学报》2010年第1期114-118,共5页Acta Anatomica Sinica

摘　　要：目的探讨过氧化物酶体增殖因子激活受体γ(PPAR-γ)、环氧合酶-2(COX-2)在子宫内膜癌中的表达及其超微结构定位。方法免疫组织化学法检测正常子宫内膜、子宫内膜增生症、子宫内膜癌组织中PPAR-γ、COX-2的表达;胶体金免疫电镜技术观察COX-2在子宫内膜癌细胞中的超微结构定位。结果免疫组织化学结果显示,1.PPAR-γ在正常子宫内膜、子宫内膜增生症中不表达,在子宫内膜癌中表达;2.COX-2在正常子宫内膜、子宫内膜增生症、子宫内膜癌3种组织中均表达,但表达不同,3组间差异有显著性(P<0.01);3.子宫内膜癌组织中PPAR-γ的表达与COX-2的表达显著相关(P<0.05)。免疫电镜结果显示,子宫内膜癌细胞的内质网和核膜上有黑色圆形集中的胶体金颗粒分布。结论PPAR-γ和COX-2可能参与子宫内膜癌的发生、发展。Objective To investigate the development and prognosis of peroxisome proliferator-activated recepor-γ（PPAR-γ） and cyclooxygenase-2 （COX-2） in endometrial carcinoma. Methods The electron microscopic immunohistochemical technique was used to observe the uhrastructure location of COX-2 protein labeled by colloidal gold in the endometrial carcinoma. Results 1. There were negative immunohistoehemieal staining signals of PPAR-γ in both the normal endometrium and hyperplasia endometrium, whereas, was positive staining in the endometrial carcinoma; 2. The intensities of the COX-2 immunohistoehemical staining were significantly statistical difference among the normal endometrium, the hyperplasia endometriu and the endometrial carcinoma（P 〈 0. 01 ） ; 3. The COX-2 labelled by colloidal gold granules was observed in the endoplasmie reticulum, nuclear membrane and nuclei in the endometrial carcinoma. Conclusion Both PPAR-γ and the COX-2 might play an important role in the development of the endometrial carcinoma.

关 键 词：激活过氧化物酶体增殖因子受体-γ 环氧合酶-2 子宫内膜癌 免疫组织化学 胶体金免疫电镜技术 人 

分 类 号：R737.33[医药卫生—肿瘤]