p16^(INK4A)在动情周期和早孕期小鼠子宫内膜的表达  

Expression of p16^(INK4A) in mouse endometrium during the estrus cycle and the early pregnancy period

作　　者：郗强[1] 朱兴春[2] 姜蓉[3] 杨戎[1] 魏莎莉[1] 张文文[1] 

机构地区：[1]重庆医科大学生殖生理学教研室,重庆400016 [2]川北医学院附属医院检验科,四川南充637000 [3]重庆医科大学干细胞与组织工程学研究室,重庆400016

出　　处：《解剖学报》2010年第1期132-136,共5页Acta Anatomica Sinica

摘　　要：目的检测肿瘤抑制基因p16INK4A在小鼠动情周期和早孕期子宫内膜中的表达,初步探讨p16INK4A在小鼠胚胎植入过程中的生物学作用。方法运用反转录-聚合酶链反应(RT-PCR)技术检测动情周期和早孕期小鼠子宫内膜p16INK4A mRNA的表达水平;用免疫组织化学和Western blotting方法检测p16INK4A蛋白的表达。结果RT-PCR显示,早孕期比动情周期p16INK4A mRNA的表达有显著增强。动情周期中动情期比其他3期表达明显增强;早孕期中,随妊娠天数的增加,p16INK4A mRNA的表达也逐渐增强,孕5d达峰值,之后又逐渐减弱。免疫组织化学和Western blotting的结果也都显示,p16INK4A蛋白在子宫内膜的表达及规律与RT-PCR的结果一致。结论p16INK4A在小鼠的胚泡植入过程中起重要作用。Objective To investigate the expression of tumor suppressor gene p16^INK4A in mouse endometrium during the estrus cycle and early pregnancy and its possible role in blastocyst implantation. Methods Reverse transcription polymerase chain reaction （RT-PCR） was used to detect the expression of p16^INK4A mRNA, immunohistochemistry and Western blotting were applied to detect p16^INK4A protein in mouse endometrium tissues during the estrus cycle and early pregnancy. Results The intensity of p16^INK4A mRNA expression in mouse early pregnancy was higher than that in the estrus cycle. Compared with the other 3 stages, the level of p16^INK4A mRNA expression at estrus was obviously higher. During the early pregnancy, the level of p16^INK4A mRNA expression increased steadily from day 2 to day 5,reaching the maximal level on day 5,then decreasing. Both immunohistochemical and Western blotting analysis showed the same results in expression patterns of p16^INK4A protein for mouse endometrium tissues as those results of RT-PCR. Conclusion p16^INK4A is involved in the embryos penetrating into the endometrial barrier.

关 键 词：P16^INK4A 子宫内膜 反转录-聚合酶链式反应 免疫组织化学 免疫印迹法 小鼠 

分 类 号：R394[医药卫生—医学遗传学]