血清载脂蛋白CⅡ含量对HDL亚类分布的影响  

Influences of apolipoprotein CII concentrations on high-density lipoprotein subclass distribution

作　　者：邢瑞青[1] 杨鲁川[1] 李俊葓[1] 李晓颖[1] 许飞[1] 傅强[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物化学与分子生物学教研室,四川成都610041

出　　处：《中国病理生理杂志》2010年第3期543-548,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30770985)

摘　　要：目的:探讨血清载脂蛋白CⅡ含量对HDL亚类分布的影响。方法:采用双向电泳-免疫印迹方法检测247例受试者血清HDL亚类组成及含量。结果:随血清apoCⅡ水平的逐渐升高,年龄、BMI、TG、TC、apoB100、apoCⅡ、apoCⅢ、apoE、preβ1-HDL、preβ2-HDL、HDL3b和HDL3a含量显著增加,HDL-C、HDL2a、HDL2b含量显著减少。随apoCⅡ和apoAⅠ水平的逐渐升高,preβ1-HDL的含量均呈增加趋势,但HDL2b的含量在同一apoCⅡ组随apoAⅠ水平的升高呈增加趋势,而在同一apoAⅠ组随apoCⅡ水平的增加呈减少趋势。随apoCⅡ/apoCⅢ比值的逐渐升高,preβ1-HDL的含量呈增加趋势,而HDL2b的含量呈减少趋势。相关分析发现,控制血清TG和TC浓度后,apoCⅡ与preβ1-HDL呈显著正相关(r=0.186,P<0.01),与HDL2b呈显著负相关(r=-0.149,P<0.05);apoAⅠ含量与所有HDL亚类呈显著正相关(r值在0.349-0.587之间,P<0.01);此外,apoCⅢ与preβ1-HDL和preβ2-HDL呈显著正相关(r分别为0.184和0.178,P<0.01);apoB100与HDL2a呈显著负相关(r=-0.102,P<0.05);apoE与HDL3a呈显著正相关(r=0.040,P<0.05)。结论:随血清apoCⅡ水平的逐渐升高,HDL亚类的颗粒呈减小趋势,提示HDL成熟代谢受阻;apoAⅠ的含量可以对抗apoCⅡ对HDL亚类分布的影响;apoCⅡ/apoCⅢ的比值亦可作为反映HDL亚类分布的指标之一。AIM： To investigate the influence of serum apolipoprotein （apo） CII concentrations on the distribution of serum high-density lipoprotein （HDL） subclasses. METHODS： Serum HDL subclasses in 247 subjects were determined by two dimensional gel electrophoresis-immunodetection. RESULTS： With the increase in serum apolipoprotein CII levels, age, BMI, the contents of TG, TC, apoB100, apoCII, apoCIII, apoE, preβ1-HDL, preβ2-HDL, HDL3b and HDL3a increased significantly, but the contents of HDL-C, HDL2a and HDL2b decreased remarkably. The contents of preβ1-HDL increased with the rise in apoCII and apoA I levels, whereas the content of HDL2b increased with the rise in serum apoA I level in the same apoC II group, but decreased with the increase in serum apolipoprotein CII level in the same apoA I group. With the increase in the ratio of apoCII/ apoCIII, the content of preβ1-HDL elevated, but the content of HDL2b decreased. The correlation analysis illustrated that the apoCII level was positively correlated with preβ1-HDL （r=0.186, P〈0.01）, but inversely correlated with HDL2b （r=-0.149, P〈0.05）. The apoA I level was positively associated with all HDL subclasses （r in the range of 0.349-0.587, P〈0.01）. In addition, the apoCIII level was positively correlated with preβ1-HDL （r=0.184, P〈0.01） and preβ2-HDL （r=0.178, P〈0.01）, while the apoE level was positively correlated with HDL3a （r=0.040, P〈0.05）. The apoB100 level was inversely correlated with HDL2a （r=-0.102, P〈0.05）. CONCLUSION： The particles of HDL show a general shift towards smaller size with the increase in apoCII levels, indicating that the maturation of HDL is abnormal. Whereas the contents of apoA I level correct the effect of apoCII on the distribution profile of HDL subclasses. The ratio of apoCII/apoCIII might also been taken as one of the indexes reflecting the distribution profile of serum HDL subclasses.

关 键 词：HDL亚类 载脂蛋白CⅡ 

分 类 号：R363[医药卫生—病理学]