氯乙烯暴露工人淋巴细胞P53基因mRNA表达  

P53 mRNA expression in peripheral lymphocytes among workers exposed to vinyl chloride

作　　者：仇玉兰[1,2] 孙品[1] 王威[1] 吴芬[1] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院劳动卫生教研室,上海200032 [2]山西医科大学公共卫生学院卫生毒理教研室

出　　处：《中国公共卫生》2010年第3期303-304,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30671740)

摘　　要：目的探讨氯乙烯(VCM)暴露工人外周血淋巴细胞P53mRNA表达的变化,分析P53基因表达与VCM暴露量以及P53基因多态的关系。方法以VCM>1年的75名工人为暴露组,以43名无VCM以及其他毒物接触史的本地志愿者为对照组,实时定量PCR嵌合荧光法(SYBR Green I染料法)检测P53基因mRNA表达量。结果VCM暴露组和对照组P53 mRNA相对表达量(2-△Ct×102)的中位数及上、下四分位数分别为0.467(0.097,1.816)和1.640(0.960,2.680),经稳健线性回归分析排除年龄因素的影响,2组表达量差异有统计学意义(χ2=53.42,P<0.000 1)。按累计接触剂量将暴露人群分为高接触组和低接触组,2组P53基因表达差异无统计学意义(P=0.966)。P53基因3个多态位点不同基因型对其mRNA表达量无明显影响(P>0.05)。结论VCM暴露会引起机体P53mRNA表达量降低。Objective To explore the relationship between vinyl chloride monomer（VCM）exposure and P53 genotype polymorphism and mRNA expression of P53 in lymphocytes among workers exposed to VCM.Methods Seventy-five occupationally VCM-exposed workers（exposure period more than one year） and 43 controls without history of exposure to VCM or other toxicants were enrolled in this study.The quantity of gene mRNA expression was detected with real-time PCR（SYBR Green I）.Results The level of P53 mRNA relative expression was 4.674（0.965,18.163） in VCM-exposed workers and 16.400（9.600,26.800） in unexposed controls.After adjustment for age,robust multiple regression indicated that the P53 mRNA expression of VCM-exposed workers was significantly lower than that of the non-exposed group（χ2=53.42,P0.0001）.The results also showed that the cumulative exposure dose and P53 genotype had no effect on mRNA expression of P53 in VCM-exposed workers.Conclusion The mRNA expression of P53 in peripheral lymphocyte is significantly decreased in VCM exposed workers compared to unexposed controls.

关 键 词：氯乙烯 实时定量PCR P53基因 MRNA表达 

分 类 号：R774[医药卫生—眼科]