口胶粘膜癌前病变及鳞癌角蛋白表达的免疫组化研究  

Immunohistochemical study of oral mucosa precancerous lesion and expression of cytokeratin of squamous cell carcinoma

作　　者：刘真子[1] 戚向敏[1] 龙彦[1] 孙善珍[1] 马伯龙[1] 凌涤生[1] 

机构地区：[1]山东医科大学附属口腔医院,250012

出　　处：《上海口腔医学》1998年第4期190-192,共3页Shanghai Journal of Stomatology

基　　金：山东省卫生厅资助!93-007

摘　　要：目的研究口腔粘膜标本中角蛋白的表达与分布。方法利用多克隆抗角蛋白抗体A(575)，采用免疫组化技术。结果从正常口腔粘膜，轻、中、重度异常增生到鳞癌角蛋白表达显著增强。A(575)在所有鳞癌标本中均为阳性，但染色强度深浅不一，且随鳞癌分化程度降低而降低，染色弱的病例其临床分期高，更易发生淋巴结转移。结论角蛋白表达的增强在强化上皮细胞中的致癌基因上起作用，这种强化的积累将加速癌前疾病的进展，因此，A575染色可能为预后提供参考依据。Objective The aim of this study was to investigate the expression and distribution of cytokeratin in oral mucosa. Methods Polyclonal cytokeratin antibody A575 and ABC immunohistochemical technique were used. Results A tendency of progressive increase of cytokeratin expression was demonstrated from normal mucosa, mild, moderate and severe dysplasia to squamous cell carcinoma. A notable overexpression was found in moderate,severe dysplasia and squamous cell carcinoma. The poor-differentiation was related to the low reaction of A575. The cases of strong reaction of A575 were more advanced stages and metastasized to lymph nodes frquently. Conclusion The overexpression of cytokeratin could play a role in potentiation the effects of genetic factors and activate oncogenes in the affected cells. The cumulative effects of the potentiation would be accelerate the progression of precancerous conditions. The reaction of A575 was correlated with clinical stages and lymph node metastasis. [

关 键 词：口腔粘膜肿瘤 癌前病变 鳞癌 免疫组化 角蛋白 

分 类 号：R739.850.2[医药卫生—肿瘤]