MMP2和MMP9在HepG2肝癌细胞系及HCC组织中表达的比较研究  

Comparative study of the expression of MMP2 and MMP9 in HepG2 cells and HCC tissues

作　　者：吴丹[1,2] 冯华[1] 赵然[1] 王天真[1] 金晓明[1] 

机构地区：[1]哈尔滨医科大学病理学教研室,黑龙江哈尔滨150081 [2]黑龙江中医药大学附属第一医院病理科,黑龙江哈尔滨150040

出　　处：《哈尔滨医科大学学报》2010年第1期29-32,共4页Journal of Harbin Medical University

基　　金：黑龙江省科技厅重大课题(ZJY04-0102)

摘　　要：目的分析MMP2和MMP9在HepG2细胞系及肝细胞癌(HCC)组织中的表达,探讨不同的基质金属蛋白酶(MMPs)与肝细胞癌侵袭转移的特异相关性。方法免疫细胞化学检测HepG2细胞系中MMP2和MMP9的表达,免疫组织化学检测38例HCC肝癌组织中MMP2和MMP9的表达。结果MMP9在HepG2细胞系的表达高于MMP2的表达。MMP9和MMP2在HCC组织中均有所表达,但MMP9的表达率84.2%(32/38)明显高于MMP2的表达52.6%(20/38),二者有显著差异(P<0.05)。结论MMP2、MMP9的表达在体内外呈现出一致性,即MMP9在体内外的表达均高于MMP2,推测可能MMP9与HCC的侵袭转移关系更为密切。Objective To investigate the expression of MMP2,MMP9 in HepG2 cells and hepatocellular carcinoma（HCC） tissues and compare their specificity to the invasion and metastasis of HCC.Methods HepG2 cells and 38 specimens of HCC tissues were assessed by immunocytochemistry and immunohistochemistry（ABC method）.Results The expression of MMP9 was higher than that of MMP2 in HepG2 cell.MMP2 and MMP9 both expressed in the HCC tissues while the positive rate of MMP9（84.2%,32/38） and the positive rate of MMP2（52.6%,20/38） had significant differences（P0.05）.Conclusion There is a good consistency in HepG2 cells and HCC tissues for MMP2,MMP9 expression,and the higher expression of MMP9 may show us that it has a closer relationship to the invasion and metastasis of HCC.

关 键 词：HEPG2细胞系 肝细胞癌 MMP2 MMP9 

分 类 号：R735.7[医药卫生—肿瘤]