内毒素对肝星状细胞醛固酮分泌及核因子-kB P65mRNA表达的影响  

Effects of endotoxin on aidosteron secretion and nuclear factor-KB P65 mRNA expression of hepatic steilate cell

作　　者：黄丽丽[1] 赵龙凤[1] 李红[1] 郝彦琴[1] 

机构地区：[1]山西医科大学第一医院感染病科,太原030001

出　　处：《中华传染病杂志》2010年第1期1-4,共4页Chinese Journal of Infectious Diseases

摘　　要：目的探讨内毒素对大鼠肝星状细胞（HSC）醛固酮的分泌及核因子-kB P65（NF—kB P65）mRNA表达的影响。方法传代培养大鼠HSC-T6，不同浓度内毒素处理细胞后分组。放射免疫法检测HSC分泌醛固酮的情况，半定量RTPCR方法检测HSC表达NF-kB P65mRNA的水平。应用方差分析、t检验和Pearson直线相关分析法进行统计学分析。结果浓度为0．01、0．1、1．0和10．0mg／L内毒素组醛固酮的分泌分别为（4．95±0．35）、（5．52±0．32）、（6．04±0．60）和（5．16±0．46）ug／L，显著高于对照组的（3．65±0．51）ug／L（t=2．9745，5．8725，6．8465，3．2065；均P〈0．05）。浓度为0．01、0．1、1．0和10．0mg／L内毒素组NF-kBP65mRNA的表达分别为0．824±0．06、1．07±0．07、1．23±0．06和0．96±0．05，也明显高于对照组的0．43±0．04（t=5．4776，6．8084，7．9382，7．5136；均P〈0．01）。10．0mg／L内毒素组醛固酮的分泌和NF—kBP65mRNA的表达均较1．0mg／L内毒素组有明显降低（t=4．3865，3．7246；均P〈0．05）。HSC中醛固酮的分泌与NF-kBP65mRNA的表达存在正相关（r=0．886，P〈0．01）。结论一定浓度范围的内毒素可以上调大鼠HSC醛固酮的分泌和NF-KBP65mRNA的表达，两者存在正相关和一定的剂量效应关系，这可能是引起肝纤维化的重要因素之一。Objective To study the effects of endotoxin on aldosteron secretion and nuclear factor-kB P65 （NF-kB P65） mRNA expression in rat hepatic stellate cell （HSC）. Methods Cultured rat HSC were treated with endotoxin of different concentrations. Aldosteron secretion of HSC was detected by radio-immunoassay. NF-kB P65 mRNA expression of HSC was determined by semiquantitative reverse transcription-polymerase chain reaction （RT-PCR）. The data were analyzed by variance analysis, t test and Pearson linear regression analysis. Results Aldosteron secretions of HSCgroups treated with0.01, 0.1, 1.0 and 10.0 mg/Lendotoxin [（4.95±0.35）, （5.52±0.32）, （6.04±0.60） and （5.16±0.46） ug/L, respectively] were all significantly higher than that in control group（3.65±0.51） ug/L （t=2. 9745, 5. 8725, 6. 8465 and 3. 2065, respectively; all P〈0.05）. NF -kB P65 mRNA expressions of HSC groups treated with 0. 01, 0. 1, 1.0 and 10. 0 mg/L endotoxin （0. 82±0. 06, 1.07±0.07, 1.23±0.06 and0.96-/0.05, respectively） were also significantly higher than that in control group 0.43±0.04 （t=5.4776, 6. 8084, 7. 9382 and 7. 5136, respectively; all P〈0.01）. Both aldosteron secretion and NF-kB P65 mRNA expression in HSC group treated with 10.0 mg/L endotoxin were significantly lower than those in HSC group treated with 1.0 mg/L endotoxin （t=4.3865, 3. 7246; both P〈0. 05）. In these treated HSC, aldosteron secretion was positively correlated with NF-kB P65 mRNA expression （r=0.886, P〈 0.01）. Conclusions Aldosteron secretion and NF-kB P65 mRNA expression in rat HSC could be up-regulated by stimulation with endotoxin, which shows a certain dose-response relationship. This may be one of the important factors of hepatic fibrosis development.

关 键 词：内毒素类 肝星状细胞 醛固酮 核因子-KB 肝硬化 RNA 信使 基因表达 

分 类 号：R575.2[医药卫生—消化系统]