毛头鬼伞子实体中甾类化合物的结构鉴定及其抑制肿瘤细胞增殖活性的研究  

Steroids from fruiting bodies of Coprinus comatus and their inhibition to tumor cell proliferation

作　　者：冯娜[1,2] 张劲松[1] 唐庆九[1] 郝瑞霞[1] 刘艳芳[1] 杨焱[1] 贾薇[1] 周帅[1] 唐传红[1] 钟建江[2] 

机构地区：[1]农业部应用真菌资源与利用重点开放实验室上海市食用菌工程技术研究中心上海市农业遗传育种重点开放实验室上海市农业科学院食用菌研究所,上海201106 [2]上海交通大学生命科学技术学院微生物代谢教育部重点实验室,上海200240

出　　处：《菌物学报》2010年第2期249-253,共5页Mycosystema

基　　金：上海市农委重点攻关项目(No农科攻字2003-3-3)

摘　　要：从毛头鬼伞子实体中分离得到4个甾类化合物,通过波谱分析,分别鉴定为麦角甾醇(1)、啤酒甾醇(2)、麦角甾醇葡萄糖甙(3)和tuberoside(4)。4个化合物均为首次从毛头鬼伞中得到。通过体外细胞毒性筛选试验,结果表明化合物4有较强的抑制人乳腺癌细胞MCF-7和狗肾细胞MDCK增殖的活性,其抑制增殖的IC50值分别为10.9μg/mL(18.4μmol/L)和5.8μg/mL(9.8μmol/L)。化合物3对MCF-7和MDCK的抑制作用则较弱,当其浓度为10.0μg/mL(17.9μmol/L)时,对MCF-7和MDCK的增殖抑制率分别为12.5%和7.5%。Four steroids were isolated from fruiting bodies of Coprinus comatus by using column chromatography.On the basis of spectral data,they were identified as ergosterol（1）,cerevisterol（2）,ergosteryl-3-O-β-D-glucopyranoside（3）,and tuberoside（4）.All these compounds were isolated from this fungus for the first time.By in vitro cytotoxicity screening,compounds 3 and 4 were found to inhibit the proliferation of MCF-7 tumor cells and MDCK cells.Compound 4 showed strong inhibition to the proliferations of MCF-7 and MDCK at IC50 of 10.9μg/mL（18.4μmol/L） and 5.8μg/mL（9.8μmol/L） respectively.Compound 3 showed weak inhibition to MCF-7 and MDCK＇s proliferations with inhibition rates of 12.5% and 7.5% respectively at 10.0μg/mL（17.9μmol/L）.

关 键 词：食药用真菌 次生代谢产物 核磁共振 细胞毒性 

分 类 号：Q946[生物学—植物学]