重组腺病毒Ad-hTERTp-HSV-TK／GCV系统对小鼠肝癌腹腔种植腹腔积液生成的影响  

Inhibitory effect of Ad-hTERTp-HSV-TK/GCV system on mice ascites production planted by liver cancer cell line

作　　者：杨强[1] 邓志华[1] 刘燕[1] 贾静[1] 郭素雅[1] 刘京龙[1] 

机构地区：[1]山西医科大学第二医院消化科,太原030001

出　　处：《肿瘤研究与临床》2010年第2期101-104,共4页Cancer Research and Clinic

基　　金：国家自然科学基金（30672405）

摘　　要：目的研究重组腺病毒Ad—hTERTp—HSV，TK／GCV系统对小鼠肝癌腹腔种植腹腔积液生成的影响及其作用机制。方法SX，近交系小鼠腹腔内接种肝癌H22细胞株后，随机分为四组，48h后分别给予相应的治疗。观察各组小鼠腹腔积液及生存时间的变化；流式细胞术测定肿瘤细胞凋亡百分率；透射电镜观察腹腔内肿瘤细胞的形态学变化。结果与Ad-hTERTp—HSV-TK组、GCV组和空白对照组三组相比，Ad—hTERTp—HSV—TK／GCV组可以显著抑制小鼠腹腔积液的生成（P〈0．01），延长生存期（P〈0．01），肿瘤细胞凋亡率为（27．12±2．12）％明显高于其他各组（P〈0．01），并且在治疗期间未发现明显不良反应。结论Ad—hTERTp—HSV—TK／GCV系统可通过诱导肝癌细胞凋亡而抑制小鼠腹腔积液生成，并延长生存期，是一种安全可行的治疗方法。Objective To observe the effect of Ad-hTERTp-HSV-TK/GCV system on malignant ascites of miee and probe into its mechanism of action. Methods The SXI inbred strain mice were injected with H22 cell line of liver cancer and were divided into 4 groups at random.The mice in each group were given corresponding treatment after 48 hours. The production of ascites and survival period were evaluated. The apoptosis rates of tumor cells were detected by FCM. Morphological changes of tumor cells were studied by electromicroscope. Results Compared with other groups, Ad-hTERTp-HSV-TK/GCV can obviously inhibit the production of aseites （P 〈0.01）, prolong the survival period （P 〈0.01）, and apoptosis rate in this group （27.12±2.12）% was significantly higher than that in other groups. No obvious side effect was found during the treatment. Conclusion Ad-hTERTp-HSV-TK/GCV system can inhibit production of ascites and prolong the survival period of mice by inducing apoptosis of hepatoma cells, which is a safe and feasible treatment for hepatoma therapy.

关 键 词：肝肿瘤 实验性 基因疗法 胸苷激酶 腺病毒 人 腹腔积液 

分 类 号：R735[医药卫生—肿瘤]