高粱EST-SSR标记的建立及其在苏丹草中的应用初探  

Establishment of sorghum EST-SSR marker and its preliminary application to sudangrass

作　　者：李杰勤[1] 王丽华[1] 詹秋文[1] 陈亚[1] 

机构地区：[1]安徽科技学院,安徽凤阳233100

出　　处：《草业科学》2010年第3期112-117,共6页Pratacultural Science

基　　金：科技部农业科技成果转化资金项目(2008GB2C300125);国家科技支撑计划项目(2008BADB3B3B10)

摘　　要：从NCBI中下载了202 325条高粱Sorghumbicolor EST序列。去除低质量和冗余的序列后,在5 661条高粱EST中共发掘出了6 663个SSR位点,出现频率是20.19%,平均分布频率是1/3.93 kb。在5 197条EST序列中,共有3 446条序列能够设计引物,占总数的66.3%。在高粱EST-SSR中,三核苷酸重复是主要的重复类型,出现最多的重复基元是GGC/GCC。在3 446对高粱EST-SSR引物中,随机选择了20对引物进行了合成。以苏丹草Sorghumsudanense722和高粱Sorghumbicolor TX623A为模板,用这20对引物进行PCR扩增,结果全部可以扩增出条带,可用率为100%,有差异的有4对,多态性比率为20%。研究结果证明了根据高粱EST建立SSR标记是有效、可行的。Total 202 325 Sorghum bicolor ESTs were downloaded from NCBI EST database. 6 663 SSRs loci within 5 661 ESTs were found out, and the emergence frequency was 20. 19%, with the average distribution frequency of 1/3.93 kb. 3 446 （66.3%） from 5 197 ESTs could be used to design primers. Trinucleotide repeat was found to be the dominant type and GGC/GCC were the most frequent motifs in sorghum EST-SSRs. 20 pairs of primers were selected to design. Using sudangrass 722 and sorghum TX623A as templates, all, the designed primers could be used to amplify the target sequences, and the usable rate was 100%, in which 4 pairs of primers were discrepant, and the polymorphism rate was 20%. Our results indicated that it was an effective and feasible approach to develop SSR markers based on sorghum ESTs.

关 键 词：高粱 苏丹草 EST-SSR 

分 类 号：S544.1[农业科学—作物学]