清洁生产驱动的弗氏链霉菌新霉素发酵过程优化策略  

Cleaner production-drived optimization strategy for neomycin fermentation with Streptomyces fradiae

作　　者：石贤爱[1,2] 李聪颖[2] 陈飞[2] 邱小明[2] 孟春[2] 郭养浩[1,2] 

机构地区：[1]福建省医疗器械与医药技术重点实验室,福建福州350002 [2]福州大学生物科学与工程学院,福建福州350108

出　　处：《福州大学学报（自然科学版）》2010年第1期147-152,共6页Journal of Fuzhou University(Natural Science Edition)

基　　金：福建省科技重大专项资助项目(2004HZ03-1)

摘　　要：新霉素的传统生产工艺导致产率低、单耗高、排污量大,造成资源利用率低,环境污染严重.经对发酵过程的分析及考察表明,弗氏链霉菌产生的胞外淀粉水解酶活力极低,不足以完成以淀粉为补料介质的充分水解,引起非可发酵性糖大量积累和残糖浓度的升高.由此确定了新霉素发酵过程优化策略,按此策略进行了新霉素发酵过程清洁生产工艺的研究,并得到如下结论:发酵120h,放罐效价从8000提高到11000U.mL-1以上;残糖浓度从3.0%下降到1.5%以下,淀粉单耗从18.5μg.U-1下降至13.5μg.U-1,同时排放COD量减少近50%,达到了"减污"、"增效"的清洁生产目的.The traditional technique for neomycin production results in low utilization rate of resources and serious environmental pollution,as the technique runs at low yield of production,high specific consumption and exorbitant pollution discharge.The investigation and analysis of the fermentation process under this traditional technique showed the activity of starch hydrolase excreted by Streptomyces fradiae in the broth was too low to hydrolyze the starch fully in bioreactor.The incomplete utility of the starch which was fed intermittent into bioreactor caused the accumulation of nonfermentable sugar in broth,and led to excessive residual sugar in broth at the end of the fermentation process.Based on these researches,a cleaner production-drived optimization strategy for neomycin fermentation was established,which included the optimization of feeding broth ingredient and feeding rate,as well as the smooth control of some process parameters.As a result,the cleaner production-drived neomycin fermentation was achieved and the goal aimed at reducing pollution and improving yield was reached,with the production concentration,the sugar concentration and the starch consumation being 11 000 U ·mL-1,1.5% and 13.5 μg · U-1 from 8 000 U ·mL-1,3.0% and 18.5 μg · U-1,respectively.At the same time,the emission COD was reduced to about 50%.

关 键 词：新霉素 发酵过程 优化策略 清洁生产 淀粉水解酶 

分 类 号：TQ464.7[化学工程—制药化工]