三角帆蚌五个野生群体线粒体DNA 16S rRNA遗传特性  

Genetic characteristics of five Hyriopsis cumingii wild populations based on mitochondrial DNA 16S rRNA sequencing

作　　者：汪桂玲[1] 李家乐[1,2] 白志毅[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 [2]上海市高校水产养殖学E-研究院,上海201306

出　　处：《生态学杂志》2010年第2期377-381,共5页Chinese Journal of Ecology

基　　金：国家重点基础研究发展计划项目(2009CB126000);国家自然科学基金项目(30871923);上海市科委地方院校能力建设项目(08390510100);上海市水产养殖重点学科建设资助项目(Y1101)

摘　　要：对中国主要淡水湖泊(鄱阳湖、洞庭湖、太湖、洪泽湖及巢湖)三角帆蚌5个野生群体的线粒体DNA 16S rRNA基因片段进行了扩增和测序,得到473bp的碱基序列,没有发现插入/缺失突变的核苷酸位点。检测到了32个多态性核苷酸位点,共7种单倍型。鄱阳湖群体的222(C→G)和325(A→G)位点,太湖群体的233(A→G)位点,巢湖群体的40(A→G)、138(A→T)和294(C→T)位点,洪泽湖群体的241(A→C)位点的变异可以作为区分群体分子遗传标记位点。洞庭湖群体未发现特异位点。在5个群体间鄱阳湖群体多态性位点、核苷酸多态性、单倍型多态性和单倍型数量4个指标都最高,表明鄱阳湖群体具有最为丰富的遗传结构,遗传变异最大,可作为三角帆蚌选育的基础群体。Hyriopsis cumingii is a major commercially important mussel for freshwater pearl culture in China. In this study,direct sequencing of mitochondrial DNA （mt DNA） 16S rRNA gene region （473 bp） was used to investigate the genetic variability of H. cumingii populations from five lakes in China. For the test individuals,no nucleotide position had gaps or insertions of base pairs,and 32 positions were variable in all the sequences with 473 base pairs. Different H. cumingii populations were indicated by different positions of genetic sequence,which could be used as the genetic markers to identify the populations,and in specific,positions 222（C→G） and 325（A→G） for Poyang Lake population,positions 233（A→G） for Taihu Lake population,positions 40（A→G）,138（A→T）,and 294（C→T） for Chaohu Lake population,and position 241（A→C） for Hongze Lake population. A total of 7 haplotypes were identified in the five populations,of which,3 unique haplotypes （Hap1,Hap2,and Hap4） were to Poyang Lake population. The level of genetic diversity was found higher in Poyang Lake population than in the other populations. Mitochondrial DNA data showed that Poyang Lake population was richer in genetic resources and had higher potentiality in breeding.

关 键 词：三角帆蚌 MTDNA 16S RRNA 遗传变异 

分 类 号：S917.4[农业科学—水产科学]