光谱法研究杀鼠剂溴鼠灵与环糊精超分子体系及分析应用  

Spectrofluorimetric Study of Supramolecular Inclusion between Rodenticide Brodifacoum and β-Cyclodextrin and Application

作　　者：段云青[1,2] 段志青[1] 闵顺耕[2] 熊艳梅[2] 段佳[2] 叶升峰[2] 王冬[2] 

机构地区：[1]山西农业大学文理学院,山西太谷030801 [2]中国农业大学理学院,北京100193

出　　处：《光谱学与光谱分析》2010年第4期1065-1069,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20575076);山西农业大学引进人才基金项目(20051223)资助

摘　　要：利用光谱法研究了伊环糊精与二代抗凝杀鼠剂溴鼠灵的超分子作用，根据Benesi-Hildebrand法确定二者的包络比为1：1，室温下包络常数为1．048×10^4L·mol^-1实验还考察了二者的包络机理，推测主要是溴鼠灵结构中的疏水基团联苯基进入了伊环糊精的疏水空腔。同时发现，超分子包络物的形成可以大大增强溴鼠灵的荧光发射，据此，建立了水溶液中测定溴鼠灵的荧光分析方法。在优化实验条件下，线性范围8．0×10^-8～4．0×10^-6mol·L^-1（r=0．9994），检出限为8．8×10^-9mol·L^-1，并成功地用于环境水中微量滨鼠灵的测定。The supramolecular interaction between fl-cyclodextrin and brodifacoum, an anticoagulant rodenticide of the second generation, was studied by spectroscopy. The results showed that brodifacoum and β-cyclodextrin could form an inclusion complex with an association constant of 1. 048× 10^4 L · mol^-1 and a 1 ： 1 stoichiometry based on Benesi-Hildebrand equation The inclusion mechanism was proposed to explain the inclusion mode. It was indicated that the hydrophobic group of brodifacoum molecule, biphenyl, entered into the cavity of β-cyclodextrin. At the same time, it was also observed the significant enhancement of fluorescence of brodifacoum after forming inclusion complex. According to the fluorescence enhancement phenomenon, a spectrofluorimetric method of detecting brodifacoum in aqueous media was established with the linear range of 8. 0 × 10^-8-4.0 × 10^-9 mol L^-1 and the correlation coefficient of 0. 999 4. The detection limit of the method was 8. 8X 10-9 mol·L^-1. The proposed method was successfully applied to determine the trace amount of brodifacoum in environment water and the recovery was in the range of 87.3% to 103.9%.

关 键 词：溴鼠灵 Β-环糊精 荧光分析法 超分子包络物 

分 类 号：S482.5[农业科学—农药学]