Rhodobacter sphaeroides偶氮还原酶三级结构同源建模与分析  

Homology modeling and analysis of three-dimensional structure of azoreductase of Rhodobacter sphaeroides

作　　者：柳广飞[1] 周集体[1] 王竞[1] 周觅[1] 

机构地区：[1]大连理工大学环境学院,辽宁大连116024

出　　处：《大连理工大学学报》2010年第2期171-175,共5页Journal of Dalian University of Technology

摘　　要：利用同源建模方法构建了Rhodobacter sphaeroides偶氮还原酶AZR的三级结构模型.结果表明,AZR为α/β型结构的黄素氧化还原蛋白,5个相互平行的β折叠形成分子中间的平面,5个α螺旋分列于平面的两侧;在β折叠的C端非共价结合的黄素单核苷酸(FMN)作为氧化还原反应中心.根据序列对齐分析,将依赖黄素的偶氮还原酶分为两个家族,但结构对比分析表明,它们具有类似的三级结构和活性区域.对AZR的结构研究为发现新的偶氮还原酶和深入研究其功能奠定了基础.The three-dimensional structure model of azoreductase AZR of Rhodobacter sphaeroides was constructed using homology modeling method. It is demonstrated that AZR is a flavodoxin adopting α/β structure with the central five-stranded parallel β-sheet flanked by five α helices. A flavin mononucleotide （FMN） prosthetic group lies on top of the molecule,acting as redox and reactive centers. Flavin-dependent azoreductases are classified into two families according to the results of sequence alignments. However,structure analysis indicates that they all possess similar three-dimensional structures and active sites. The study of AZR＇s structure lays a foundation for finding new azoreductase gene and deeply understanding AZR＇s function.

关 键 词：偶氮还原酶 同源建模 黄素氧化还原蛋白 

分 类 号：Q518.2[生物学—生物化学]