反相高效液相色谱法同时测定饮料中10种添加剂  

Gr-HPLC method for simultaneous determination of 10 additives in beverage

作　　者：余明池[1] 汪道发[1] 

机构地区：[1]安徽省铜陵市疾病预防控制中心,安徽铜陵244000

出　　处：《中国卫生检验杂志》2010年第2期241-242,278,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立梯度洗脱-反相高效液相色谱法同时测定饮料中柠檬黄、苋菜红、胭脂红、日落黄、亮兰、安赛蜜、糖精钠、苯甲酸、山梨酸和咖啡因等10种添加剂的分析方法。方法：样品经过脱气,调节pH,经定量滤纸和0.45μm微孔滤膜过滤后,以甲醇-0.02 mol/L乙酸铵为流动相,梯度洗脱,反相C18柱分离,二极管阵列检测10种添加剂成分含量。结果：线性关系良好,回归系数均大于0.9995,各组分相对标准偏差小于6.7%,加标平均回收率为82%-103%。结论：该法同时测定饮料中10种添加剂成分,快速准确,重现性好,适合于不含蛋白质饮料中色素、防腐剂、甜味剂和咖啡因的检测。Objective：To establish a gradient elution RP-HPLC method for simultaneous determination of 10 additives in beverages.Methods：After sample degassing,regulate pH and filtrated through quantitative filter paper and 0.45 μm microporous membrane,the 10 additives including lemon yellow,amaranth,carmine red cochineal,sun set yellow,light blue,Acesulfame-K,sodium saccharin,benzoic acid,sorbic acid and caffein have been separated by reversed-phase C18 column with methanol-0.02 mol/L ammonium acetate as mobile phase with gradient elution and detected with diode array detector.Results：The method had good linear relationship with regression coefficient greater than 0.9995,the RSDs were less than 6.7%,and the average recoveries were 82%-103%.Conclusion：The method is fast and accurate with good reproducibility.So it is suitable for the determination of preservatives,sweeteners and caffeine in the beverages without protein.

关 键 词：梯度洗脱 反相高效液相色谱 饮料 添加剂 

分 类 号：O657.72[理学—分析化学]