荧光定量PCR检测在宫颈癌筛查中的应用  

Quantitative analysis 13 types of high risk HPV DNA in cervical lesions by fluorescence quantitative PCR

作　　者：童永清[1] 刘蓓[2] 李艳[1] 顾剑[1] 贺玉娟[2] 李锋[1] 

机构地区：[1]武汉大学人民医院检验科,湖北430060 [2]武汉科技大学附属天佑医院病理科,湖北430064

出　　处：《国际检验医学杂志》2010年第2期125-126,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨荧光定量聚合酶链反应（FQ—PCR）检测宫颈病变人乳头瘤病毒（HPV）13种高危型DNA的可行性，为检测宫颈组织中HPV的感染提供有效的方法。方法选择197例阴道细胞学检测异常的宫颈脱落细胞，使用FQPCR方法检测13种高危犁HPV DNA，同时取宫颈病变处组织进行组织病理学检查。结果329例样本中HPV 13种高危型阳性者218例，阳性率为66．26％：CT值范围在13～40之间，平均为（26±2．31）；组织病理学检测186例阳性，阳性率为56．53％，经x^2检验，差异有统计学意义（x^2=6．566，P=0．0104）；阴性标本为Undet。结论FQ-PCR方法检测宫颈病变中13种高危型HPV DNA感染，具有快速、简便、灵敏度高、特异性强等优点，对宫颈癌的普查和治疗有指导意义。Objective Quantitative detected 13 types of high risk HPV DNA in cervical lesions by FQ-PCR,in order to establish a quick and sensitive test to detect HPV in cervical lesions. Methods 13 types of high risk HPV DNA were detected in 329 cases of cervical lesions by FQ-PCR. HPV was also detected by histopathologieal examination. Results The positive rate of 13 types of high risk HPV DNA was 66.26% by FQPCR. The Ct（Threshold Cycle）value scope between 13-40,and the average value was 26±2.31. There were Under in negative cases. The positive rate of HPV was 56. 53% by bistopathologieal examination. There was a significant difference between FQ-PCR and histopathological examination（x^2 =6. 566, P=0. 010 4）. Conclusion FQPCR is a valuable method for quantitative detection 13 types of high risk HPV DNA in cervical lesions. There are simple,the sensitivity to be high fast, specificity strong and so on merits, has the guiding sense to cervical cancer＇s general survey and the treatment.

关 键 词：聚合酶链反应 人乳头瘤病毒16 宫颈肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]