利用TCT技术和PCR-RDB法筛查宫颈高度病变  

Detecting high grade cervical intraepithelial neoplasia by TCT and PCR-RDB

作　　者：肖克林[1] 吴丽娟[1] 王敏[2] 梅平[3] 王丁[3] 周克元[4] 

机构地区：[1]深圳市宝安区妇幼保健院检验科,518133 [2]中山大学达安基因股份有限公司科研部,510080 [3]广东省人民医院病理医学部,510080 [4]广东医学院生物化学与分子生物学教研室,524023

出　　处：《国际检验医学杂志》2010年第2期127-128,130,共3页International Journal of Laboratory Medicine

摘　　要：目的评价TCT技术、PCR-RDB法检测女性宫颈高度病变的价值。方法利用TCT技术和PCR-RDB法分别检测宫颈上皮细胞病变及HPV感染情况,以组织病理检查结果进行评价。结果 TCT技术检测宫颈高度病变的敏感性、特异性、阳性预测值和阴性预测值分别为46.2%(95%CI,19.1%～73.3%)、98.0%(95%CI,96.3%～99.7%)、54.5%(95%CI,25.1%～84.0%)和97.2%(95%CI,95.2%～99.3%),PCR-RDB法分别为100.0%(95%CI,100.0%～100.0%)、58.6%(95%CI,52.5%～64.8%)、11.2%(95%CI,5.5%～17.0%)和100.0%(95%CI,100.0%～100.0%),两者联合后分别为100.0%(95%CI,100.0%～100.0%)、98.0%(95%CI,96.2%～99.7%)、77.3%(95%CI,59.8%～94.8%)和100.0%(95%CI,100.0%～100.0%)。结论 TCT技术和PCR-RDB法检测宫颈高度病变具有很好的互补性,两者联合应用对于筛查宫颈高度病变有很高的应用价值。Objective To evaluate the diagnostic accuracy of TCT and PCR-RDB in detecting high grade cervical intrepithelial neoplasis. Methods Participants＇ cervical epithelium specimens were collected for cervical cytology test by TCT and HPV test by PCR-RDB,then,the two methods＇ accuracy were evaluated by histopathology result. Results The sensitivity, specificity, positive predictive value and negative predictive value of TCT for detection ≥CIN2 were 46.2 % （95 % CI, 19. 1%- 73.3 % ）, 98.0% （95 % CI,96.3%-99.7%） ,54.5%（95%CI,25.1%-84.0%） and 97.2%（95%CI,95.2%-99.3%）,respectively. Those of PCR-RDB were 100.0%（95%CI,100.0%-100.0%）,58. 6%（95%CI,52. 5%-64. 8V0）,11. 2%（95%CI,5. 5%-17.0%） and 100.0% （95%CI,100.0%-100.0%） ,respectively. Those of combined TCT with PCR-RDB were 100.0%（95%CI,100.0%-100.0%）, 98.0%（95%CI,96. 2%-99. 7%）,77. 3%（95%CI,59. 8%-94.8%） and 100.0%（95%CI,100. 0%-100.0%）,respectively. Conclusion There is good collaboration between TCT and PCR-RDB,and there is high detection valuation when combine TCT with PCR RDB.

关 键 词：聚合酶链反应 人乳头瘤病毒16 宫颈肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]