睡眠剥夺对大鼠脑5-羟色胺代谢及行为的影响  

The effects of prolonged sleep deprivation on brain serotonin metabolism and behavior in rats

作　　者：郑乐颖[1] 季红光[1] 王海明[1] 潘祥福[1] 阮芳铭[1] 

机构地区：[1]第二军医大学海医系,上海200433

出　　处：《中国行为医学科学》1998年第4期256-257,292,共3页Chinese Journal of Behavioral Medical Science

基　　金：解放军"九五"科研基金

摘　　要：目的探讨长时间睡眠剥夺（sleepdeprivation，SD）对大鼠下丘脑和脑干5-羟色胺（5-HT）代谢及行为的影响。方法用水上转盘（dish-over-water）睡眠剥夺模型对大鼠分别进行24h、48h、72h睡眠剥夺，观察睡眠剥夺后下丘脑、脑干两脑区5-HT代谢的变化，同时利用旷场试验观察大鼠主动行为的改变。结果睡眠剥夺大鼠的旷场试验得分较非剥夺大鼠高（P＜0.05），但随着时间的延长出现下降趋势;5-HT向5-羟吲哚乙酸（5-HIAA）转化在经过24h睡眠剥夺后显著增高（P＜0.01），但在48h开始下降，72h后出现大幅下降（P＜0.01）。结论长时间睡眠剥夺对大鼠行为的影响是一个由兴奋到抑制的过程，这种变化可能与下丘脑和脑干的5-HT代谢有关。Objective The effects of prolonged sleeep deprivation (SD) on hypothalamus and brain stem 5-hydroxytryptamine (5-HT)metabolism and behavior in rats were studied. Method Dish-over-watermodel was used in order to observe the effects of 24hr, 48hr and 72hr sleep deprivation on hypothalamus and brain stem 5-HT metabolism in Sprague-Dawley rats, and the active behavior of rats was also examined by open field test (OFT). Results The OFT scores weresignificantly increased after 24hr, 48hr and 72hr SD, but the scores after 72hr SD was lower than those after 24hr and 48hr SD(P <0. 01). The5-hydroxyindoleacetic acid/5-hydroxytryptamine (5-HIAA/5-HT) ratio were significantly increased after 24hr SD in the hypothalamus and brainstem, but the ratio were decreased after 48hr and 72hr SD. Conclusion The activity behavior in sleep deprived rats exhibted an pattern from activity to depression, which may he related to thtl 5-HT metabolic changes in hypothalamus and brain stem.

关 键 词：睡眠剥夺 行为 5-羟色胺 5-羟吲哚乙酸 大鼠 

分 类 号：R749[医药卫生—神经病学与精神病学]