肝癌、肺癌及唾液腺肿瘤中铜锌超氧化物歧化酶定位及意义  

LOCATION OF COPPER-ZINC SUPEROXIDE DISMUTASE IN TISSUES FROM HEPATOCELLULAR CARCINOMA,LUNG CANCER AND SALIVARY GLAND TUMORS

作　　者：李秀霞[1,2] 白石山[1,2] 赵利珍[1,2] 王越先 

机构地区：[1]内蒙古医学院病理教研室 [2]解放军第253医院

出　　处：《内蒙古医学院学报》1998年第4期218-221,共4页Acta Academiae Medicinae Neimongol

摘　　要：目的：探讨肿瘤与ＣｕＺｎＳＯＤ的关系。方法：用ＡＢＣ免疫组化法对１９例肝细胞癌及癌旁肝组织；４５例肺癌；７２例唾液腺良、恶性肿瘤及正常肝、肺、唾液组织切片中ＣｕＺｎＳＯＤ定位及初步定量。结果：ＣｕＺｎＳＯＤ分布在阳性细胞质和（或）核内，肝癌中ＣｕＺｎＳＯＤ阳性率（３１５８％）及染色强度明显＜正常肝组织＜癌旁组织。肺癌阳性率为５３３３％，肺鳞癌ＣｕＺｎＳＯＤ主要分布在细胞质，而肺腺癌主要分布在细胞质和核，二者之间有显著区别（Ｐ＜００１）。肺小细胞未分化癌阳性率为９０９％，显著低于其它类型肺癌（Ｐ＜００１）。唾液腺良、恶性肿瘤之间无明显差异。肝、肺及唾液腺三种不同组织来源的肿瘤ＣｕＺｎＳＯＤ含量差异很大。结论：肝、肺及唾液腺三种不同组织来源的肿瘤中ＣｕＺｎＳＯＤ含量差异很大，故不同组织类型的肿瘤抗氧化能力亦不同。其ＣｕＺｎＳＯＤ免疫定位及定量显示出病理学意义。Objective:To seek the relationship between the tumors and copper-zinc superoxide dismutase(CuZnSOD).Methods:Immunohistochemical location and initial quantitative analysis of CuZnSOD in tissues from 19 hepatocellular carcinoma(HCC)and around HCC,45 carcinoma of lung and 72 benign and malignant salivary tumors were studied by ABC method,the normal tissues of these organs were served as control.Results:In positive cells CuZnSOD was observed in nuclei or/and plasm.The positive ratio (31 58%)and dyeing intensity of HCC was lower than that of normal liver cells,but the highest is in the tissues around HCC.The positive ratio of CuZnSOD was 53 33% in lung cancer.CuZnSOD was mainly observed in cytoplasm from the squamous cells carcinoma of lung and in nuclei and plasm from the adenocarcinoma of lung,the difference in the two groups being statistically significant(P<0 01).The positive ratio of the small cell undifferentiated carcinoma of lung was 9 09% and was significantly lower than that of other groups of lung cancer(P<0 01).There was no significant difference between benign and malignant salivary tumors.There were obvious differences of CuZnSOD content among different tissues from liver,lung and salivary gland tumors.Conclusion:The amounts of CuZnSOD in malignant tumors from liver,lung and salivary gland were very different,so that the antioxidant capability of tumors from different type of tissuse are different .So immunohistochemical location and quantitative analysis have pathological importance.

关 键 词：肝癌 肺癌 唾液腺肿瘤 免疫测定 SOD 

分 类 号：R735.702[医药卫生—肿瘤] R734.202[医药卫生—临床医学]