一个氨基甙类抗生素致聋家系线粒体DNA序列分析  

Mitochondrial DNA Sequence Analysis of One Family With Aminoglycoside Antibiotics Induced Deafness

作　　者：黄鹰[1] 陆明华[1] 张丽珊[1] 周晓雷[1] 邱定红[1] 龚嫦虹 王为未[1] 

机构地区：[1]南京铁道医学院生物学教研室

出　　处：《南京铁道医学院学报》1998年第4期240-242,共3页Journal of Nanjing Railway Medical College

基　　金：江苏省卫生厅资助

摘　　要：目的：通过对一个氨基甙类抗生素致聋家系１１名成员的线粒体ＤＮＡ进行分析，探讨该病的遗传方式及与线粒体ＤＮＡ突变的关系。方法：对１１名成员外周血线粒体ＤＮＡ进行ＰＣＲ－ＲＦＬＰ及测序分析。结果：８例标本的ＰＣＲ－ＲＦＬＰ结果异常，测序表明其均存在１５５５位点Ｃ－Ｔ突变。结论：氨基甙类抗生素致聋与线粒体ＤＮＡ突变有关，该家系呈典型母系遗传。Objective:The mtDNA of 11 members from a family with aminoglycoside antibiotics-induced deafness(AAID) was analyzed to abserve the inheritance type of the disease and it's relation to the mtDNA mutation.Methods:The mtDNA of peripheral blood cells from 11 family members was amplified by polymerase chair reaction-restriction fragment length poly-morphism (PCR-RFLP) and direct sequencing was then employed.Results:The results of 8 members were abnormal and show a C to T mutation at 1555 site.Conclusion:The Occurrence of AAID is associated with the mtDNA mutation and it has typical material hereditary character.

关 键 词：氨基甙类 抗生素 药源性聋 线粒体DNA 基因突变 

分 类 号：R764.43[医药卫生—耳鼻咽喉科]