赖氨酸转基因大米与亲本大米在五指山小型猪回肠中的消化代谢比较  

Comparative research on digestibility of lysine-rich genetically modified rice and its parental rice in Wuzhishan mini-pig

作　　者：胡贻椿[1] 李敏[1] 朴建华[1] 杨晓光[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050

出　　处：《卫生研究》2010年第1期32-35,共4页Journal of Hygiene Research

基　　金：国家重点基础研究发展计划"973"项目(No.2007CB109207);转基因生物新品种培育重大专项课题(No.2008ZX08011-005)

摘　　要：目的对赖氨酸转基因大米与其亲本大米中蛋白质和氨基酸在猪回肠的消化代谢情况进行比较研究。方法选择近交系五指山小型猪为实验动物,通过外科手术将T型瘘管安置于小型猪回肠末端,给其饲喂赖氨酸转基因大米和亲本大米,收集小型猪回肠末端的消化产物进行分析,采用酪蛋白饲料法测定内源性氨基酸的排出量,从而比较两种大米蛋白质和氨基酸的表观消化率和真消化率。结果赖氨酸转基因大米与亲本大米的蛋白质表观消化率和真消化率都没有显著差异;各种氨基酸的消化率结果显示,除半胱氨酸的表观消化率外,其他氨基酸的表观消化率和真消化率都没有显著差异。结论赖氨酸转基因大米与亲本大米在五指山小型猪体内的蛋白质和氨基酸消化代谢基本没有差异,均能很好地被消化吸收。Objective To study the digestibility of protein and amino acids in lysine-rich genetically modified （GM） rice and its parental rice.Methods Eight Wuzhishan Mini-pig Inbred-line as the experimental animals,T-cannulas were equipped at their terminal ileums by surgical operation.Six of them,which were well-healed and with good appetite,were fed with lysine-rich genetically modified rice and its parental rice,and digesta of each pig was collected and analyzed respectively for comparing their apparent digestibility （AD） and true digestibility （TD）.The endogenous amino acids losses were determined by casein diet method.Results The AD and TD of protein are very similar in the two types of rice.There are no statistical differences in the AD of all amino acids except for Cysteine,and there are no statistical differences in the TD of all 18 amino acids.Conclusion The lysine-rich GM rice has no statistical difference with its parental rice in terms of protein and amino acid digestion and absorption in Wuzhishan mini-pigs.

关 键 词：赖氨酸 转基因大米 五指山小型猪 表观消化率 真消化率 

分 类 号：TS201.6[轻工技术与工程—食品科学] Q591.2[轻工技术与工程—食品科学与工程]